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Molecular characterization of Leptospira spp. strains isolated from small rodents in Croatia

Published online by Cambridge University Press:  17 February 2003

N. TURK ,
Z. MILAS ,
J. MARGALETIC ,
V. STARESINA ,
A. SLAVICA ,
N. RIQUELME-SERTOUR ,
E. BELLENGER ,
G. BARANTON  and
D. POSTIC
N. TURK
Affiliation:
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Z. MILAS
Affiliation:
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine University of Zagreb, Zagreb, Croatia
J. MARGALETIC
Affiliation:
Department of Forest Protection and Game, Faculty of Forestry University of Zagreb, Zagreb, Croatia
V. STARESINA
Affiliation:
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine University of Zagreb, Zagreb, Croatia
A. SLAVICA
Affiliation:
Chair of Game Biology & Pathology, School of Veterinary Medicine University of Zagreb, Zagreb, Croatia
N. RIQUELME-SERTOUR
Affiliation:
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France
E. BELLENGER
Affiliation:
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France
G. BARANTON
Affiliation:
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France
D. POSTIC
Affiliation:
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France
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Abstract

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We report the isolation and characterization of 16 Leptospira spp. strains isolated from small rodents captured in 11 different regions of inland Croatia. Large NotI and SgrAI restriction fragment allowed us to assign 10 isolates to the serovar istrica, 5 isolates to the serovar tsaratsovo and 1 isolate to the serovar lora. The phylogenetic analysis conducted from the sequences of the first 330 bp from the 16S rDNA gene revealed that the strains belonged to three different species, L. borgpetersenii, L. kirschneri and L. interrogans. Carrier rates in eight rodent species varied from 0 to 71·4%. Mus musculus showed the highest infection level and confirmed its role as a major reservoir of the serogroup Sejroë. For the first time we reported the occurrence of serovars tsaratsovo and lora in Croatia.

Type
Research Article
Information
Epidemiology & Infection , Volume 130 , Issue 1 , February 2003 , pp. 159 - 166
Copyright
2003 Cambridge University Press