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Molecular characterization of a virulence-associated epitope on the lipopolysaccharide of Legionella pneumophila serogroup 1

Published online by Cambridge University Press:  15 May 2009

J. H. Helbig ,
P. C. Lück ,
Y. A. Knirel ,
W. Witzleb  and
U. Zähringer
J. H. Helbig
Affiliation:
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der TU Dresden, Dürerstr. 24, D-01307 Dresden, Germany
P. C. Lück
Affiliation:
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der TU Dresden, Dürerstr. 24, D-01307 Dresden, Germany
Y. A. Knirel
Affiliation:
Forschungsinstitut Borstel, Institut für Experimented Biologie und Medizin, D-23845 Borstel, Germany N D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
W. Witzleb
Affiliation:
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der TU Dresden, Dürerstr. 24, D-01307 Dresden, Germany
U. Zähringer
Affiliation:
Forschungsinstitut Borstel, Institut für Experimented Biologie und Medizin, D-23845 Borstel, Germany
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For identification of lipopolysaccharide (LPS)-associated epitopes of Legionella pneumophila serogroup 1, LPS of strain Philadelphia 1 was investigated using monoclonal antibodies (MAbs). The O-specific chain of LPS is a homopolymer of 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-D-glycero-L-galacto-nonulosonic acid. At least four immunoaccessible epitopes were recognized by different MAbs on the intact LPS. After O-deacetylation of LPS, the reactivity of one of the MAbs (MAb 3/1) was lost, indicating thus that the corresponding epitope is associated with the 8-O-acetyl group. Since the reactivity pattern of the MAb 3/1 is identical with those of the MAb 2 which was considered as a virulence marker for serogroup 1, this epitope may be involved in mediating virulence in L. pneumophila. Four MAbs specific to strains of serogroup 1 other than the monoclonal subtype Philadelphia recognized epitopes on the O-deacetylated LPS of strain Philadelphia 1 and, therefore, the virulence-associated epitope blocks recognition of the immunodeterminants that are accessible on the intact LPS of the strains lacking this epitope.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 1 , August 1995 , pp. 71 - 78
Copyright
Copyright © Cambridge University Press 1995

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