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Molecular characterization and antibiotic susceptibility of Vibrio cholerae non-O1

Published online by Cambridge University Press:  15 May 2009

A. Dalsgaard ,
O. Serichantalergs ,
C. Pitarangsi  and
P. Echeverria
A. Dalsgaard
Affiliation:
Laboratory of Fish Diseases, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark
O. Serichantalergs
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
C. Pitarangsi
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
P. Echeverria
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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A collection of 64 clinical and environmental Vibrio cholerae non-O1 strains isolated in Asia and Peru were characterized by molecular methods and antibiotic susceptibility testing. All strains were resistant to at least 1 and 80% were resistant to two or more antibiotics. Several strains showed multiple antibiotic resistance (≥ three antibiotics). Plasmids most often of low molecular weight were found in 21/64 (33%) strains. The presence of plasmids did not correlate with antibiotic resistance or influence ribotype patterns. In colony hybridization studies 63/64 (98%) V. cholerae non-O1 strains were cholera toxin negative, whereas only strains recovered from patients were heat-stable enterotoxin positive. Forty-seven Bgl I ribotypes were observed. No correlation was shown between ribotype and toxin gene status. Ribotype similarity was compared by cluster analysis and two main groups of 13 and 34 ribotypes was found. Ribotyping is apparently a useful epidemiological tool in investigations of V. cholerae non-O1 infections.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 51 - 63
Copyright
Copyright © Cambridge University Press 1995

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