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A modified metabolic inhibition test for the titration of poliovirus neutralizing antibodies

Published online by Cambridge University Press:  15 May 2009

Marjorie V. Mussett ,
Mileva Dimic ,
Nermin Ege  and
J. O'H. Tobin
Marjorie V. Mussett
Affiliation:
Department of Biological Standards, National Institute for Medical Research, Mill Hill, London, N.W.7
Mileva Dimic
Affiliation:
Biological Standards Control Laboratory, Medical Research Council Laboratories, Hampstead, London, N.W.3
Nermin Ege
Affiliation:
Biological Standards Control Laboratory, Medical Research Council Laboratories, Hampstead, London, N.W.3
J. O'H. Tobin
Affiliation:
Biological Standards Control Laboratory, Medical Research Council Laboratories, Hampstead, London, N.W.3
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A modification of the metabolic inhibition test for titrating poliomyelitis antibody is described. The method depends on the substitution of galactose for glucose in the initial medium to eliminate colour change during the period of virus activity. Neutralization is detected by the addition of glucose at the end of the period, when the usual colour changes associated with this type of test occur. A statistical analysis of the results obtained is given.

This method can also be used for titrating antibodies to other enteric viruses.

Thanks are due to Dr Hélène Mair, Dr D. R. Gamble and Dr A. D. Macrae for strains of enteroviruses, and to Miss Patricia Jerram, for valuable technical assistance in the early days of this work.

Type
Research Article
Information
Journal of Hygiene , Volume 59 , Issue 3 , September 1961 , pp. 295 - 302
Copyright
Copyright © Cambridge University Press 1961

References

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