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The microbiology of selected retail food products with an evaluation of viable counting methods

Published online by Cambridge University Press:  19 October 2009

M. H. Greenwood ,
E. F. C. Coetzee ,
B. M. Ford ,
P. Gill ,
W. L. Hooper ,
S. C. W. Matthews ,
S. Patrick ,
J. V. S. Pether  and
R. J. D. Scott
M. H. Greenwood
Affiliation:
Public Health Laboratory, Poole General Hospital, Poole BH15 2JB
E. F. C. Coetzee
Affiliation:
Public Health Laboratory, Glyde Path Road, Dorchester DT1 1XD
B. M. Ford
Affiliation:
Public Health Laboratory, Poole General Hospital, Poole BH15 2JB
P. Gill
Affiliation:
Public Health Laboratory, Glyde Path Road, Dorchester DT1 1XD
W. L. Hooper
Affiliation:
Public Health Laboratory, Poole General Hospital, Poole BH15 2JB
S. C. W. Matthews
Affiliation:
Public Health Laboratory, Odstock Hospital, Salisbury SP2 8BJ
S. Patrick
Affiliation:
Public Health Laboratory, Odstock Hospital, Salisbury SP2 8BJ
J. V. S. Pether
Affiliation:
Public Health Laboratory, Taunton and Somerset Hospital, Musgrove Park Branch, Taunton TA1 5DB
R. J. D. Scott
Affiliation:
Public Health Laboratory, Taunton and Somerset Hospital, Musgrove Park Branch, Taunton TA1 5DB
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Summary

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In an inter-laboratory survey, the pour plate, surface spread, agar droplet and spiral plate methods were used in parallel with the surface drop method for enumeration of micro-organisms in foods. Good agreement was obtained between all surface methods of enumeration, but there was poor agreement between molten agar methods and the surface drop method.

A total of 1143 samples of food that were ready for consumption at the point of retail sale were examined. Eight types of food products were chosen: meat pasties, sausage rolls, real-cream slices, synthetic-cream slices, mayonnaise-based coleslaws, faggots, patés and continental sausages. The results of this survey suggest that the upper limit for an acceptable viable count should vary according to the food product. Salmonellae were not isolated on any occasion. Potentially harmful organisms were not isolated at levels expected to constitute a public health hazard.

Information concerning the nature of the product, the total viable count, the presence or absence of pathogenic, toxigenic or indicator organisms, the spectrum of the bacterial flora and the relative predominance of each organism must all be considered when assessing the microbiological acceptability of retail ‘ready to eat’ products.

Type
Research Article
Information
Journal of Hygiene , Volume 92 , Issue 1 , February 1984 , pp. 67 - 77
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Baird-Parker, A. C. (1962). An improved diagnostic and selective medium for isolating coagulase-positive staphylococei. Journal of Applied Bacteriology 25, 12–19.Google Scholar
Barrell, R. A. E. & Watkinson, J. M. (1981). The bacteriology of cooked meats. Environmental Health 89 (6), 148–151.Google Scholar
Bassett, W. H., Kurtz, J. B. & Moore, B. (1978). The hygienic significance of bacterial counts on sliced cooked meats. Environmental Health 86 (5), 100–103.Google Scholar
Davis, J. G. (1969). Microbiological standards for foods: II. Laboratory Practice 18, 830–844.Google Scholar
Gilchrist, J. E., Campbell, J. E., Donnelly, C. B., Peeler, J. T. & Delaney, J. M. (1973). Spiral plate method for bacterial determination. Applied Microbiology 25, 244–252.CrossRefGoogle ScholarPubMed
Goldenberg, N. (1964). Food hygiene: standards in manufacture, retailing and catering. Royal Society of Health Journal 84, 105–201.CrossRefGoogle ScholarPubMed
Hedges, A. J., Shannon, R. & Hobbs, R. P. (1978). Comparison of the precision obtained in counting viable bacteria by the Spiral Plate Maker, the Droplette, and the Miles and Misra methods. Journal of Applied Bacteriology 45, 57–65.CrossRefGoogle ScholarPubMed
Hobbs, B. C. & Gilbert, R. J. (1970). Microbiological standards for food: public health aspects. Chemistry and Industry, pp 215–219.Google Scholar
Jarvis, B., Lach, V. H. & Wood, J. M. (1977). Evaluation of the spiral plate maker for the enumeration of microorganisms in foods. Journal of Applied Bacteriology 44, 821–827.Google Scholar
Kramer, J. M. & Gilbert, R. J. (1978). Enumeration of microorganisms in food: a comparative study of five methods. journal of Hygiene 81, 151–159.CrossRefGoogle ScholarPubMed
Kramer, J. M., Kendall, M. & Gilbert, R. J. (1979). Evaluation of the spiral plate and laser colony counting techniques for the enumeration of bacteria in foods. European Journal of Applied Microbiology and Biotechnology 6, 280–299.Google Scholar
Miles, A. A. & Misra, S. S. (1938) The estimation of the bactericidal power of the blood. Journal of Hygiene 38, 732–749.Google ScholarPubMed
Sharpe, A. N., Dyett, E. J., Jackson, A. K. & Kilsby, D. C. (1972). Technique and apparatus for rapid and inexpensive enumeration of bacteria. Applied Microbiology 24, 4–7.CrossRefGoogle ScholarPubMed
Sharpe, A. N. & Kilsby, D. C. (1971). A rapid inexpensive bacterial count technique using agar droplets. Journal of Applied Bacteriology 34, 435–440.Google Scholar
Thatcher, F. S. & Clark, D. S. (1968). Microorganisms in Foods. Vol. I. Their Significance and Methods of Enumeration, pp. 66–69. Canada: University of Toronto Press.Google Scholar
Thatcher, F. S. & Clark, D. S. (1974). Sampling for Microbiological Analysis: Principles and Specific Applications. Canada: University of Toronto Press.Google Scholar
Tiwari, N. P. & Kadis, V. W. (1981). Microbiological quality of some delicatessen meat products. Journal of Food Protection 44, 821–827.CrossRefGoogle ScholarPubMed
Wehr, H. M. (1978). Microbiological standards for food: attitudes and policies of state governments. Food Technology 32, 63–65.Google Scholar