Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-22T15:15:41.707Z Has data issue: false hasContentIssue false

Methods for the isolation of Aeromonas hydrophila and Plesiomonas shigelloides from faeces

Published online by Cambridge University Press:  19 October 2009

S. E. Millership
Affiliation:
Public Health Laboratory and Department of Microbiology, Whipps Cross Hospital, Leytonstone, London Ell 1NR
B. Chattopadhyay
Affiliation:
Public Health Laboratory and Department of Microbiology, Whipps Cross Hospital, Leytonstone, London Ell 1NR
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two solid selective media, xylose deoxycholate citrate agar (XDCA) and bile salts brilliant green agar (BBG) and an enrichment broth–alkaline peptone water, were evaluated for the isolation of Aeromonas hydrophila and Plesiomonas shigelloides. Alkaline peptone water and XDCA are useful for recovery of Aeromonas but not Plesiomonas, whereas BBG is satisfactory for both organisms.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Bhat, P., Shanthakumri, S. & Rajan, D. (1974). The characterization and significance of Plesiomonas shigelloides and Aeromonas hydrophila isolated from an epidemic of diarrhoea. Indian Journal of Medical Research, 10511060Google ScholarPubMed
Catsaras, M. & Buttiaux, R. (1965). Les Aeromonas dans les matières fécales humaines. Annales de l'institut Pasteur de Lille 16, 8588.Google Scholar
Chatterjee, B. D.). & Neogy, K. N. (1972). Studies on Aeromonas and Plesiomonas species isolated from cases of choleraic diarrhoea. Indian Journal of Medical Research 60, 520524.Google ScholarPubMed
Cooper, R. G. & Brown, G. W. (1968). Plesiomonas shigelloides in South Australia. Journal of Clinical Pathology 21, 715718.CrossRefGoogle ScholarPubMed
Cowan, S. T. (1979 a). Manual for the Identification of Medical Bacteria, 2nd ed. (ed. Cowan, S. T. and Steel, K. J.), p. 177. Cambridge: Cambridge University Press.Google Scholar
Cowan, S. T. (1979 b). Manual for the Identification of Medical Bacteria, 2nd ed. (ed. Cowan, S. T. and Steel, K. J.), p. 170. Cambridge: Cambridge University Press.Google Scholar
Davis, W. A. II, Kane, J. G. & Gauagusi, V. F.. (1978). Human Aeromonas infections: a review of the literature and a case report of endocarditis. Medicine 57, 267277.CrossRefGoogle Scholar
Furniss, A. L., Lee, J. V. & Donovan, T. J. (1978). The Vibrios. Public Health Laboratory Monograph Series 11, 20.Google Scholar
Gracey, M., Burke, V. & Rohinson, J. (1982). Aeromonas-associated gastroenteritis. Lancet ii, 13041306.CrossRefGoogle Scholar
Lautrop, H. (1961). Aeromonas hydrophila isolated from human faeces and its possible pathological significance. Acta Pathologica et Microbiologica Scandinavica (Suppl.) 51, 299301.Google Scholar
Millership', S. E., Curnow, S. R. & Chattopadhyay, B. (1983). Faecal carriage rate of Aeromonas hydrophila. Journal of Clinical Pathology 36, 920923.CrossRefGoogle ScholarPubMed
Paučková, V. & Fukalová, A. (1968). Occurrence of Aeromas hydrophila and Aeromonas shigelloides in feces. Zentralblatt für Bakteriologie. I. Abteilung: Originale 206, 212216.Google ScholarPubMed
Rogol, M., Sechter, I., Grinberg, L. & Gerichter, Ch. B.. (1979). Pril-xylose-ampicillin agar. A new selective medium for the isolation of Aeromonas hydrophila. Journal of Medical Microbiology 12, 229231.CrossRefGoogle Scholar
Rosner, R.. (1964). Aeromonas hydrophila as the etiologic agent in a ease of severe gastroenteritis. American Journal of Clinical Pathology 42, 402404.CrossRefGoogle Scholar
Schubert, R. H. W. (1977). Üeber den Nachweis von Plesiomonas shigelloides Habs und Schubert, 1962, und ein Elektivmedium, den Inositol-Brillant grün-Gallesalz-Agar. Ernst-Rodenwaldt-Archiv 4, 97103.Google Scholar
Shread, P., Donovan, T.J. & Lee, J. V. (1981). A survey of the incidence of Aeromonas in human faeces. Society of General Microbiology Quarterly 8, 1.Google Scholar
Stokes, E. J. & Ridgway, G. L. (eds) (1980). Clinical Bacteriology, 5th ed. p. 215. London: Arnold.Google Scholar
Von Graevenitz, A. & Bucher, C. (1983). Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces. Journal of Clinical Microbiology 17, 1621.CrossRefGoogle ScholarPubMed
Von Graevenitz, A. & Mensch, A. H. (1968). The genus Aeromonas in human bacteriology. Report of 30 cases and review of the literature. New England Journal of Medicine 278, 245249.CrossRefGoogle ScholarPubMed
Von Graevenitz, A. & Zinterhofer, L. (1970). The detection of Aeromonas hydrophila in stool specimens. Health Laboratory Sciences 7, 124127.Google ScholarPubMed