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Mechanisms of resistance of staphylococci grown in plasma to polymorph bactericidins

Published online by Cambridge University Press:  19 October 2009

Deboye O. Kolawole
Deboye O. Kolawole
Affiliation:
Department of Microbiology, University of Ife, Ile-Ife, Nigeria
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Summary

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The mechanisms whereby staphylococcal strains grown in plasma assume increased resistance to polymorph bactericidins were investigated. Observations reported here showed that cultural conditions could determine the path of conversion to resistance. Staphylococcal strains and mutants lacking either free coagulase or clumping factor or both all showed enhanced resistance after 10 h incubation in plasma proteins, thus giving no clear indication that these factors were involved in the interactions. In fact, prolonged incubation in bovine serum albumin (22 h) and ordinary broth medium (24 h) also resulted in increased resistance. A distinction between staphylococcal factors interacting specifically with plasma proteins and such non-specific conversions was obtained in two different ways. Stripping of a hypothetical surface protein by treatment with trypsin or 2 M potassium bromide rendered plasma- but not 24 h-broth organisms susceptible, indicating protein coating of plasma-grown organisms. Also free coagulase-positive strains and mutants incubated in plasma for 30 min were converted while those lacking both or possessing clumping factor alone were not. It therefore appears that one of the mechanisms of acquiring resistance involves a rapid interaction between staphylococcal-free coagulase and fibrinogen, resulting in the deposition of fibrin or fibrin derivatives on the bacterial surface.

Type
Research Article
Information
Journal of Hygiene , Volume 92 , Issue 2 , April 1984 , pp. 183 - 192
Copyright
Copyright © Cambridge University Press 1984

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