Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-19T12:30:07.194Z Has data issue: false hasContentIssue false
  • English
  • Français

Measurement of endotoxins with the limulus test in burned patients

Published online by Cambridge University Press:  15 May 2009

R. J. Jones  and
E. A. Roe
R. J. Jones
Affiliation:
M. R. C. Industrial Injuries and Burns Unit, Birmingham Accident Hospital, Bath Row, Birmingham B15 1NA
E. A. Roe
Affiliation:
M. R. C. Industrial Injuries and Burns Unit, Birmingham Accident Hospital, Bath Row, Birmingham B15 1NA
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

High titres of endotoxin as measured by the Limulus test were usually found in burned patients who had raised body temperatures, and were colonized with gram-negative bacteria; also some infected patients showed raised endotoxin without a raised temperature.

Patients vaccinated with an antipseudomonas vaccine rarely showed endotoxin in their plasma but occasional plasma samples from vaccinated patients had a high titre of endotoxin which appeared unrelated to infection or to a raised temperature in the patient.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 1 , August 1979 , pp. 151 - 156
Copyright
Copyright © Cambridge University Press 1979

References

Babb, J. R., Bridges, K., Jackson, D. M., Lowbury, E. J. L. & Ricketts, C. R. (1977). Topical chemoprophylaxis: trials of silver phosphate chlorhexidine, silver sulphadiazine and povidone iodine preparations. Burns 3, 65–71.CrossRefGoogle Scholar
Caridis, D. T., Reinhold, W. B., Woodruff, P. W. H. & Fire, J. (1972) Endotoxaemia in man. Lancet i, 1381–5.CrossRefGoogle Scholar
Clark, I. A. (1978). Does the endotoxin cause both the disease and parasite death in acute malaria and babesiosis? Lancet ii, 75–7.CrossRefGoogle Scholar
Davis, B. D., Lilly, H. A. & Lowbury, E. J. L. (1966), Gram-negative bacilli in burns. Journal of Clinical Pathology 22, 634,CrossRefGoogle Scholar
Jones, R. J. (1974). Topical chemoprophylaxis against infection of burns. In Symposium on the Treatment of Burns from the Proceedings of the Dutch Burns Association (ed. Hermans, R.), Beverwijk, Hollandpp. 21–26.Google Scholar
Jones, R. J., Roe, E. A. & Dyster, R. E. (1975). Detection of endotoxins. Limulus test in burned and unburned miceinfected with different species of Gram-negative bacteria. Journal of Hygiene 75, 99–112.CrossRefGoogle Scholar
Jones, R. J., Roe, E. A., Lowbury, E. J. L., Miler, J. M. & Spilsbury, J. F. (1976). A new pseudomonas vaccine: preliminary trials on human volunteers. Journal of Hygiene 76, 429–39.CrossRefGoogle ScholarPubMed
Jones, R. J., Roe, E. A. & Gupta, J. L. (1978). Low mortality in burned patients in a pseudomonas vaccine trial. Lancet ii, 401–3.CrossRefGoogle Scholar
Lowbury, E. J. L. (1976). Prophylaxis and treatment for infection of burns. British Journal of Hospital Medicine 15, 566–72.Google Scholar
Miler, J. M., Spilsbury, J. F., Jones, R. J., Roe, E. A. & Lowbury, E. J. L. (1977). A new polyvalent pseudomonas vaccine. Journal of Medical Microbiology 10, 19–27.CrossRefGoogle ScholarPubMed
Reinhold, R. B. & Fine, J. (1971). A technique for quantitative measurement of endotoxins in human plasma. Proceedings of the Society for Experimental Biology and Medicine 137, 334–40.CrossRefGoogle ScholarPubMed
Roitt, I. M. (1975). In ‘Essential Immunology’. Oxford, London, Edinburgh and Melbourne; Blackwell's ScientificPublications.Google Scholar
YinE, T. E, T., Galanos, C., Kinsky, S., Bradshaw, R. W., Wessler, S., Lunderity, O., & Sarmento, M. E. (1972). Picogram-sensitive assay for endotoxin. Gelation of Limulus polyphemus blood-cell lysate maintained by purified lipopolysaccharide and lipid A from Gram-negative bacteria. Biochimica et biophysica acta 261, 284–9.Google Scholar