Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-20T01:39:07.007Z Has data issue: false hasContentIssue false
  • English
  • Français

Mannose-resistant adhesion of motile Aeromonas to INT407 cells and the differences among isolates from humans, food and water

Published online by Cambridge University Press:  15 May 2009

Y. Nishikawa ,
T. Kimura  and
T. Kishi
Y. Nishikawa
Affiliation:
Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences, Tojo-cho, Tennoji, Osaka 543, Japan
T. Kimura
Affiliation:
Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences, Tojo-cho, Tennoji, Osaka 543, Japan
T. Kishi
Affiliation:
Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences, Tojo-cho, Tennoji, Osaka 543, Japan
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Aeromonas has been recognized as an important enteropathogen, but factors related to its virulence have not been clarified. For most enteric pathogens, attachment is a prerequisite for infection and for the effective delivery of toxins to the intestinal epithelial cells. We examined a total of 273 strains isolated from stool, food and environmental specimens by an assay for mannose-resistant adhesion to INT407 cells in vitro. Seventeen of the 102 faecal isolates were adhesive strains with more than 10 bacteria adhering per cell, while only 2 of the 118 isolates from foods and river water adhered to the cells (P < 0·001). It is possible that the adhesion might serve as a marker for discrimination between the pathogenic and nonpathogenic isolates. The 8 highly adhesive strains with more than 20 adhering organisms per cell were scrutinized for the mechanism of adhesion. No correlation was apparent between the adhesion to INT407 cells and hydrophobicity. It was noted that fucose inhibited the adhesion of four strains as well as haemagglutination by them. Electronmicroscopic studies showed the presence of flexible and curvilinear fimbriae in only 2 of the 8 highly adhesive strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 1 , August 1991 , pp. 171 - 179
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Echeverria, P, Sack, RB, Blacklow, NR, Bodhidatta, P, Rowe, B, McFarland, A. Prophylactic doxycycline for travelers' diarrhea in Thailand. Am J Epidemiol 1984; 120: 912–21.CrossRefGoogle ScholarPubMed
2.Burke, V, Gracey, M, Robinson, J, Peck, D, Beaman, J, Bundell, C. The microbiology of childhood gastroenteritis: Aeromonas species and other infective agents. J Infect Dis 1983; 148: 68–74.CrossRefGoogle ScholarPubMed
3.Rahman, AFMS, Willoughby, JMT. Dysentery-like syndrome associated with Aeromonas hydrophila. Br Med J 1980; 281: 976.CrossRefGoogle ScholarPubMed
4.Echeverria, P, Blacklow, NR, Sanford, LB, Cukor, GC. Travelers' diarrhea among American Peace Corps volunteers in rural Thailand. J Infect Dis 1981; 143: 767–71.CrossRefGoogle ScholarPubMed
5.Gracey, M, Burke, V, Robinson, J, Masters, PL, Stewart, J, Pearman, J. Aeromonas spp. in travelers' diarrhoea. Br Med J 1984; 289: 658.CrossRefGoogle Scholar
6.Agger, WA, McCormick, JD, Gurwith, MJ. Clinical and microbiological features of Aeromonas hydrophila-associated diarrhea. J Clin Microbiol 1985; 21: 909–13.CrossRefGoogle ScholarPubMed
7.Cumberbatch, N, Gurwith, MJ, Langston, C, Sack, RB, Brunton, JL. Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infect Immun 1979; 23: 829–37.CrossRefGoogle ScholarPubMed
8.Nishikawa, Y, Kishi, T. Isolation and characterization of motile Aeromonas from human, food and environmental specimens. Epidemiol Infect 1988; 101: 213–23.CrossRefGoogle ScholarPubMed
9.Figura, N, Marri, L, Verdiani, S, Ceccherini, C, Barberi, A. Prevalence, species differentiation, and toxigenicity of Aeromonas strains in cases of childhood gastroenteritis and in controls. J Clin Microbiol 1986; 23: 595–9.CrossRefGoogle ScholarPubMed
10.Millership, SE, Curnow, SR, Chattopadhyay, B. Faecal carriage rate of Aeromonas hydrophila. J Clin Pathol 1983; 36: 920–3.CrossRefGoogle ScholarPubMed
11.Pitarangsi, C, Echeverria, P, Whitmire, R, Tirapat, C, Formal, S, Dammin, GJ, Tingtalapong, M. Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand. Infect Immun 1982; 35: 666–73.CrossRefGoogle ScholarPubMed
12.Asao, T, Kozaki, S, Kato, K, Kinoshita, Y, Otsu, K, Uemura, T, Sakaguchi, G. Purification and characterization of an Aeromonas hydrophila hemolysin. J Clin Microbiol 1986; 24: 228–32.CrossRefGoogle ScholarPubMed
13.Stelma, GN Jr, Johnson, CH, Spaulding, P. Evidence for the direct involvement of β-hemolysin in Aeromonas hydrophila enteropathogenicity. Curr Microbiol 1986; 14: 71–7.CrossRefGoogle Scholar
14.Morgan, DR, Johnson, PC, DuPont, HL, Satterwhite, TK, Wood, LV. Lack of correlation between known virulence properties of Aeromonas hydrophila and enteropathogenicity for humans. Infect Immun 1985; 50: 62–5.CrossRefGoogle ScholarPubMed
15.Freter, R. Association of enterotoxigenic bacteria with the mucosa of the small intestine: mechanisms and pathogenic implications. In: Ochterlony, O, Holmgren, J, Craig, J, Barua, D. eds. Cholera and related diarrheas. Basel. Switzerland: Karger, 1978; 155–70.Google Scholar
16.Satterwhite, TK, Evans, DG, DuPont, HL, Evans, DJ. Role of Escherichia coli colonisation factor antigen in acute diarrhea. Lancet 1978; ii: 181–4.CrossRefGoogle Scholar
17.Scaletsky, ICA, Silva, MLM, Trabulsi, LR. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984; 45: 534–6.CrossRefGoogle ScholarPubMed
18.Knutton, S, Baldwin, T, Williams, PH, McNeish, AS. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 1989; 57: 1290–8.CrossRefGoogle ScholarPubMed
19.Lindahl, M, Faris, A, Wadström, T, Hjertén, S. A new test based on ‘salting out’ to measure relative surface hydrophobicity of bacterial cells. Biochim Biophys Acta 1981; 677: 471–6.CrossRefGoogle ScholarPubMed
20.Chopra, AK, Houston, CW, Genaux, CT, Dixon, JD, Kurosky, A. Evidence for production of an enterotoxin and cholera toxin cross-reactive factor by Aeromonas hydrophila. J Clin Microbiol 1986; 24: 661–4.CrossRefGoogle ScholarPubMed
21.Honda, T, Sato, M, Nishimura, T, Higashitsutsumi, M, Fukai, K, Miwatani, T. Demonstration of cholera toxin-related factors in cultures of Aeromonas species by enzyme-linked immunosorbent assay. Infect Immun 1985; 50: 322–3.CrossRefGoogle ScholarPubMed
22.Ljungh, Å, Popoff, M, Wadström, T. Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains. J Clin Microbiol 1977; 6: 96–100.CrossRefGoogle ScholarPubMed
23.Mathewson, JJ, Cravioto, A. HEp-2 cell adherence as an assay for virulence among diarrheagenic Escherichia coli. J Infect Dis 1989; 159: 1057–60.CrossRefGoogle ScholarPubMed
24.Callister, SM, Agger, WA. Enumeration and characterization of Aeromonas hydrophila and Aeromonas caviae isolated from grocery store produce. Appl Environ Microbiol 1987; 53: 249–53.CrossRefGoogle ScholarPubMed
25.Toule, G, Murphy, O. A study of bacterial contaminating refrigerated cooked chicken; their spoilage potential and possible origin. J Hyg 1978; 81: 161–9.CrossRefGoogle Scholar
26.Clerc, P, Sansonetti, PJ. Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation. Infect Immun 1987; 55: 2681–8.CrossRefGoogle ScholarPubMed
27.Robins-Browne, RM. Traditional enteropathogenic Escherichia coli of infantile diarrhea. Rev Invect Dis 1987; 9: 28–53.CrossRefGoogle ScholarPubMed
28.Baloda, SB, Faris, A, Krovacek, K. Cell-surface properties of enterotoxigenic and cytotoxic Salmonella enteritidis and Salmonella typhimurium: studies on hemagglutination, cell-surface hydrophobicity, attachment to human intestinal cells and fibronectin-binding. Microbiol Immunol 1988; 32: 447–59.CrossRefGoogle ScholarPubMed
29.Honda, T, Kahn, MMA, Takeda, Y, Miwatani, T. Grouping of enterotoxigenic Escherichia coli by hydrophobicity and its relation to hemagglutination and enterotoxin productions. FEMS Microbiol Lett 1983; 17: 273–6.CrossRefGoogle Scholar
30.Nishikawa, Y, Kishi, T, Iida, T, Yanagisawa, N. Cell surface hydrophobicity of motile Aeromonas from human, food and environmental specimens. Annual Report of Osaka City Inst Public Health Environ Sciences 1989; 51: 55–8.Google Scholar
31.Evans, DJ Jr, Evans, DG, DuPont, HL. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun 1979; 23: 336–46.CrossRefGoogle ScholarPubMed
32.Carrello., A, Silburn, KA, Budden, JR, Chang, BJ. Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells. J Med Microbiol 1988; 26: 19–27.CrossRefGoogle ScholarPubMed
33.Honda, T, Kasemsuksakul, K, Oguchi, T, Kohda, M, Miwatani, T. Production and partial characterization of pili on non-O1 Vibrio cholerae. J Infect Dis 1988; 157: 217–8.CrossRefGoogle ScholarPubMed
34.Owen, P. Outer membrane proteins: their role in virulence. In: Owen, P, Foster, TJ, ed. Immunochemical and molecular genetic analysis of bacterial pathogens. Amsterdam: Elsevier, 1988; 33–7.Google Scholar
35.Kozaki, S, Asao, T, Kamata, Y, Sakaguchi, G. Characterization of Aeromonas sobria hemolysin by use of monoclonal antibodies against Aeromonas hydrophila hemolysins. J Clin Microbiol 1989; 27: 1782–6.CrossRefGoogle ScholarPubMed
36.Clark, RB, Knoop, FC, Padgitt, PJ, Hu, DH, Wong, JD, Janda, JM. Attachment of mesophilic aeromonads to cultured mammalian cells. Curr Microbiol 1989; 19: 97–102.CrossRefGoogle Scholar
37.Parry, SH, Rooke, DM. Adhesins and colonization factors of Escherichia coli. In: Sussman, M. ed. The virulence of Escherichia coli. London: Academic Press, 1985; 111–3.Google Scholar