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A longitudinal study of Staphylococcus hyicus colonization of vagina of gilts and transmission to piglets

Published online by Cambridge University Press:  15 May 2009

H. C. Wegener  and
E. W. Skov-Jensen
H. C. Wegener*
Affiliation:
National Veterinary Laboratory, Department of Microbiology, Copenhagen, Denmark
E. W. Skov-Jensen
Affiliation:
Staby-Ulfborg-Tim Veterinarians, Ulfborg, Denmark
*
*Address for reprints/correspondence: H. C. Wegener, National Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen, Denmark.
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High Staphylococcus hyicus colonization rates were found in vaginal samples of healthy breeding sows and in skin samples of their offspring. Twenty-two different phage types were identified among the 720 isolates of S. hyicus examined. Two to 13 different phage types were isolated per herd. Phage typing, as well as characterization of about 10% of the isolates by plasmid profiles and antibiogram patterns, showed that, several different clones of S. hyicus could be present simultaneously in vagina of gilts and also on skin of piglets. Generally isolates from the vagina of one animal were identical as regards to phage types, plasmid profiles, and antibiogram patterns during the entire investigation period. Isolates from the skin of piglets were of the same type as their mothers, indicating that vertical transmission had taken place. S. hyicus strains isolated from the skin of piglets within 24 h after birth were identical to strains isolated 3 weeks after birth from the same litter, indicating that the vaginal strains became part of a stable skin flora.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 3 , December 1992 , pp. 433 - 444
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.L'Ecuver, C.Exudative epidermitis in pigs. Clinical studies and preliminary transmission trials. Can J Comp Med Vet Sci 1966; 30: 9–16.Google Scholar
2.Penny, RHC, Muirhead, MR. Skin. In: Leman, AD, Straw, RD. Gloek, WL, Mengeling, WL, Penny, RHC, Scholl, E., eds. Diseases of swine. Ames, Iowa, U.S.A.: Iowa State University Press, 1986: 82–101.Google Scholar
3.Phillips, WE, King, RE, Kloos, WE. Isolation of Staphylococcus hyicus subsp. hyicus from a pig with septic polyarthritis. Am J Vet Res 1979; 41: 274–6.Google Scholar
4.Onet, GE, Pommer, JL. Staphylococcus hyicus abortion in a Sow. J Amer Vet Med Assn 1991; 199: 362–3.CrossRefGoogle Scholar
5.Hunter, D, Todd, JN, Larkin, M. Exudative epidermitis of pigs. Br Vet J 1970: 126: 225–9.CrossRefGoogle ScholarPubMed
6.Stuker, G, Betschinger, HU. Staphylococcus hyicus: Kulturell-biochemische characterisierung serologische typisierung und pathogenitatsnachweis im tierversuch. Zentralbl Veterinärmed (B) 1976; 23: 733–43.Google Scholar
7.Amtsberg, G. Unterzuchungen zur vorkommen von Staphylococcus hyicus beim schwein bzw. Staphylococcus epidermidis biotvp 2 bei anderen tierarten. Dtsch Tierärtztl Wochenschr 1978; 85: 385–9.Google Scholar
8.Wegener, HC. Staphylococcus hyicus epidemiology and virulence in relation to exudative epidermitis in pigs. Ph.D. thesis, Royal Veterinary and Agricultural University, Copenhagen, Denmark 1992.Google Scholar
9.Taylor, DJ. Pig diseases. Foxton, Cambridge: The Burlington Press Ltd, 1983.Google Scholar
10. Pigletter Authors. Greasy pig (disease review). Int Pigletter 1991; 11: 30–2.Google Scholar
11.Devriese, LA. Isolation and Identification of Staphylococcus hyicus. Am J Vet Res 1977; 38: 787–92.Google ScholarPubMed
12.Devriese, LA, Schleifer, KH, Adegoke, GO. Identification of coagulase-negative staphylococci from farm animals. J Appl Bacteriol 1985; 58: 45–55.CrossRefGoogle ScholarPubMed
13.Lachica, RVF, Genigeorgis, C, Hoeprich, PD. Metachromatic agar-diffusion methods for detecting staphyloeoccal nuclease activity. Appl Microbiol 1971; 21: 585–7.CrossRefGoogle ScholarPubMed
14.Faller, A, Schleifer, KH. Modified oxidase and benzidene tests for separation of Staphylococci from Micrococci. J Clin Microbiol 1981; 13: 1031–5.CrossRefGoogle Scholar
15.Devriese, LA, H´jek, V. Identification of pathogenic staphylococci isolated from animals and foods derived from animals. J Appl Bacteriol 1980; 49: 1–11.CrossRefGoogle ScholarPubMed
16.Hebert, GA, Crowder, CG, Hancock, GA, Jarwis, WR, Thornsberry, C. Characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family Micrococcaceae. J Clin Microbiol 1988; 26: 1939–49.CrossRefGoogle ScholarPubMed
17.Holmes, DS, Quigley, M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 1981; 114: 193–7.CrossRefGoogle ScholarPubMed
18.Macrina, FL, Kopecko, DJ, Jones, KR, Ayers, DJ, McCowen, SM. A multiple plasmidcontaining Eschericia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978; 1: 417–20.CrossRefGoogle Scholar
19.Threlfall, EJ, Rowe, B, Ferguson, JL, Ward, LR. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986; 97: 419–26.CrossRefGoogle ScholarPubMed
20.Lautrop, H, Høiby, N, Bremmelgaard, A, Korsager, B. Bakteriologiske undersegelser. Copenhagen, Aarhus, Odense, Denmark: FADL, 1976:Google Scholar
21.Casals, JB, Pringler, N. Antibacterial sensitivity testing using Neo-Sensistabs. Taastrup, Denmark: Rosco Diagnostica, 1991.Google Scholar
22.Amtsberg, G, Bollwahn, W, Hazem, S, Jordan, B, Schmidt, U. Bakteriologische und tierexperimentelle untersuchungen zur atiologischen bedeutung von Staphylococcus hyicus beim nassendem ekzem des schweines. Dtsch Tierarztl Wochenschr 1973; 80: 521–3.Google Scholar