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The lethal effect of cotton-wool lipid on tubercle bacilli in acid conditions and its prevention by surface-active agents

Published online by Cambridge University Press:  15 May 2009

P. D'arcy Hart ,
J. E. Lovelock  and
T. Nash
P. D'arcy Hart
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London, N. W. 7
J. E. Lovelock
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London, N. W. 7
T. Nash
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London, N. W. 7
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Long-chain fatty acids are not appreciably bactericidal to tubercle bacilli at neutral pH, but become so even in slightly acid solution. In this way tubercle bacilli, normally resistant to 0·1 N hydrochloric acid (pH 1·0), are rapidly killed at this pH if traces of fatty acid are present, such as can be found inside test tubes that have been plugged with cotton wool and sterilized by dry heat.

The lethal effect is largely prevented by non-ionic, cationic, and to a lesser extent anionic detergents, which probably take up the fatty acid into the micelles so that it can no longer attack the bacilli.

The various fatty acids identified in the deposit from heated cotton wool were tested individually and found to be bactericidal. They comprised the usual C12 to C18 saturated acids and also oleic acid. The latter, however, showed no particular lethal toxicity in excess of that shown by, for example, palmitic acid. Shorter chained fatty acids than those represented in the deposit were less lethal.

We wish to thank Dr A. T. James for the chromatographic analyses and for advice; Dr R. J. W. Rees for carrying out counts of total acid-fast bacilli in two experiments; Messrs. Robinson & Sons Ltd, Chesterfield, for information on the industrial processes involved in the preparation of cotton wool; Mr E. J. Latham for advice on preparing glassware; and Dr J. Marks for helpful criticism of the manuscript.

Type
Research Article
Information
Journal of Hygiene , Volume 60 , Issue 4 , December 1962 , pp. 509 - 525
Copyright
Copyright © Cambridge University Press 1962

References

Becher, P. (1959). Non-ionic surface-active compounds. I. Critical micelle concentrations of water-soluble ether-alcohols. J. phys. Chem. 63, 1675.CrossRefGoogle Scholar
Britton, H. T. S. (1942). Hydrogen Ions, 3rd ed., vol. 1, pp. 301, 303, 308. London: Chapman & Hall.Google Scholar
Colowick, S. P. & Kaplan, N. O. (1955). Methods in Enzymology, vol. 1, p. 141. New York: Academic Press.Google Scholar
Cornforth, J. W., Hart, P. D'arcy, Nicholls, G. A., Rees, R. J. W. & Stock, J. A. (1955). Antituberculous effects of certain surface-active polyoxyethylene ethers. Brit. J. Pharmacol. 10, 73.Google ScholarPubMed
Davis, B. D. & Dubos, R. J. (1947). The binding of fatty acids by serum albumin, a protective growth factor in bacteriological media. J. exp. Med. 86, 215.CrossRefGoogle ScholarPubMed
Davson, H. & Danielli, J. F. (1952). The Permeability of Natural Membranes. Cambridge University Press.Google Scholar
Drew, W. F. (1942). Growth of small numbers of tubercle bacilli, H37, in Long's liquid synthetic medium and some interfering factors. J. Bact. 44, 149.CrossRefGoogle Scholar
Drea, W. F. (1944). Antibacterial effects of various organic substances upon the H 37 strain of human tubercle bacilli in a simple synthetic medium. J. Bact. 48, 547.CrossRefGoogle Scholar
Dubos, R. J. (1947). The effect of lipids and serum albumin on bacterial growth. J. exp. Med. 85, 9.CrossRefGoogle ScholarPubMed
Dubos, R. J. (1948). The effect of sphingomyelin on the growth of tubercle bacilli. J. exp. Med. 88, 73.CrossRefGoogle ScholarPubMed
Dubos, R. J. (1950). The effect of organic acids on mammalian tubercle bacilli. J. exp. Med. 92, 319.CrossRefGoogle ScholarPubMed
Dubos, R. J. & Davis, B. D. (1946). Factors affecting the growth of tubercle bacilli in liquid media. J. exp. Med. 83, 409.CrossRefGoogle ScholarPubMed
Dubos, R. J. & Middlebrook, G. (1947). Media for tubercle bacilli. Amer. Rev. Tuberc. 56, 334.Google ScholarPubMed
Fenner, F., Martin, S. P. & Pierce, C. H. (1949). The enumeration of viable tubercle bacilli in cultures and infected tissues. Ann. N. Y. Acad. Sci. 52, 751.CrossRefGoogle ScholarPubMed
Hart, P. D'arcy & Lovelock, J. E. (1958). Protection of living cells against acid by certain non-ionic and amphoteric soaps. Nature, Lond., 182, 304.CrossRefGoogle Scholar
Hartley, G. S. & Runnicles, D. F. (1938). The determination of the size of paraffin-chain salt micelles from diffusion measurements. Proc. Roy. Soc. A, 168, 420.Google Scholar
Hirsch, J. G. (1954). The resistance of tubercle bacilli to the bactericidal action of benzalkonium chloride (Zephiran). Amer. Rev. Tuberc. 70, 312.Google Scholar
Hoeber, R. (1945). Physical Chemistry of Cells and Tissues. London: Churchill.Google Scholar
Marks, J. & Trollope, D. R. (1960). A study of the ‘anonymous’ mycobacteria. II. Drug sensitivity; pathogenicity; hypersensitivity. Tubercle, Lond., 41, 127.CrossRefGoogle ScholarPubMed
Matijević, E. & Pethica, B. A. (1958). The heats of micelle formation of sodium dodecyl sulphate. Trans. Faraday Soc. 54, 587.CrossRefGoogle Scholar
Miles, A. A. & Misra, S. S. (1938). The estimation of the bactericidal power of the blood. J. Hyg., Camb., 38, 732.Google ScholarPubMed
Pollock, M. R. (1948). Unsaturated fatty acids in cotton-wool plugs. Nature, Lond., 161, 853.CrossRefGoogle ScholarPubMed
Pollock, M. R. (1949). The effects of long-chain fatty acids on the growth of Haemophilus pertussis and other organisms. Symp. Soc. exp. Biol. no. 3, 193.Google Scholar
Salton, M. R. J. (1950). The bactericidal properties of certain cationic detergents. Aust. J. Sci. Res. B, 3, 45.Google Scholar
Salton, C. R., Nishihara, H., Golden, F., Hoyt, A., Guss, C. O. & Kloetzel, M. C. (1950). The bactericidal effect of surface-active agents on tubercle bacilli. Publ. Hlth Rep., Wash., 65, 1588.Google Scholar
Wright, H. D. (1934). A substance in cotton wool inhibitory to the growth of the pneumococcus. J. Path. Bact. 38, 499.CrossRefGoogle Scholar
Yeglan, D. & Budd, V. (1951). The growth and enumeration of mycobacteria in transparent agar medium. Amer. Rev. Tuberc. 64, 81.Google Scholar