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Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies – an epidemiological tool

Published online by Cambridge University Press:  19 October 2009

I. D. Watkins ,
J. O'H. Tobin ,
P. J. Dennis ,
W. Brown ,
R. Newnham  and
J. B. Kurtz
I. D. Watkins
Affiliation:
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE
J. O'H. Tobin
Affiliation:
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE
P. J. Dennis
Affiliation:
Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts SP4 0JG
W. Brown
Affiliation:
Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts SP4 0JG
R. Newnham
Affiliation:
Dept of Virology, John Radcliffe Hospital, Oxford OX3 9DU
J. B. Kurtz
Affiliation:
Dept of Virology, John Radcliffe Hospital, Oxford OX3 9DU
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Summary

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A panel of 10 monoclonal antibodies was used to subgroup 326 strains of Legionella pneumophila serogroup 1. All but two strains could be classified into three major subgroups named after their representative strains Pontiac 1, Olda and Bellingham 1. Of the 50 isolates from patients, 44 representing 32 separate incidents were of the Pontiac subgroup. This subgroup was also found in 16 of 18 buildings epidomiologically associated with Legionnaires' Disease. In contrast, strains of the Olda subgroup predominated in buildings where no infections had occurred. In 9 of the 11 incidents where isolates were available from at least one patient as well as from the suspected environmental source, the monoclonal antibody reaction patterns of strains from patients were identical to those of one or more of their environmental counterparts.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 2 , October 1985 , pp. 211 - 216
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Gosting, L. H., Cabrian, K., Sturge, J. C. & Goldstein, L. C. (1984). Identification of a species specific antigen in L. pneumophila by a monoclonal antibody. Journal of Clinical Microbiology. 20, 1031–1035.CrossRefGoogle Scholar
Joly, J. R., Ches, Y. Y. & Ramsay, D. (1983). Serogrouping and subtyping of L. pneumophila with monoclonal antibodies. Journal of Clinical Microbiology 18, 1040–1046.CrossRefGoogle ScholarPubMed
Joly, J. R. & Winn, W. (1984). Correlation of subtypes of L. pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources. Journal of Infectious Diseases 150, 667–671.CrossRefGoogle Scholar
Kohler, G. & Mustein, C. (1970). Derivation of specific antibody-producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6, 511–519.CrossRefGoogle Scholar
McKinney, R. M., Thacker, L., Wells, D. E., Wong, M. C., Jones, W. J. & Bibb, W. F. (1983). Monoclonal antibodies to L. pneumophila serogroup 1: possible applications in diagnostic tests and epidemiological studies. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene Abteil 1 Originale A. 255, 91–95.CrossRefGoogle Scholar
Plouffe, J., Para, A., Hackman, B., Webster, L. & Maher, W. (1984). Nosocomial Legionnaires' Disease: different attack rates associated with two strains of Legionella pneumophila serogroup 1. In Legionella. Proceedings of the 2nd International Symposium (ed. Thornberry, C., Balows, A., Feeley, J. C. and Jakunbowski, E.) pp. 216–218. American Society of Microbiology, Washington, D.C.Google ScholarPubMed
Reingold, A. L., Thomason, B. M., Brake, B. J., Thacker, L., Wilkinson, H. W. & Kuritsky, J. N. (1984). Legionella pneumophila in the United States: the distribution of serogroups and species causing human pneumonia. Journal of Infectious Diseases 149, 819.CrossRefGoogle Scholar
Tam, M. R., Buchanan, T. M., Sandstrom, E. G., Homes, K. K., Knapp, J. S., Siadak, A. W. & Knowinski, R. C. (1982). Serological classification of N. gonnorrhoea with monoclonal antibodies. Infection and Immunology 36, 1042–53.CrossRefGoogle Scholar
Thomason, B. M. & Bibb, W. F. (1984). Uses of absorbed antisera for demonstration of antigenic variation among strains of L. pneumophila serogroup 1. Journal of Clinical Microbiology 19, 794–797.CrossRefGoogle Scholar
Watkins, I. D. & Tobin, J. O'H. (1983). Studies with Legionella pneumophila serogroup 1 monoclonal antibodies. Communicable Diseases Report. 83/40.Google Scholar
Watkins, I. D. & Tobin, J.O'H. (1984). Studies with monoclonal antibodies to Legionella species: in ‘Legionella’ Proceedings of the 2nd International Symposium (ed. Thornsberry, C., Balows, A., Feeley, J. C. and Jakunbowski, W. J.), pp. 250–262. American Society Microbiology, Washington, D.C.Google Scholar