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Legionella pneumophila: comparison of isolation from water specimens by centrifugation and filtration

Published online by Cambridge University Press:  19 October 2009

R. J. Brindle ,
P. J. Stannett  and
R. N. Cunliffe
R. J. Brindle
Affiliation:
Public Health Laboratory, Level 6/7, John Radcliffc Hospital, Headington, Oxford 0X3 9DU
P. J. Stannett
Affiliation:
Public Health Laboratory, Level 6/7, John Radcliffc Hospital, Headington, Oxford 0X3 9DU
R. N. Cunliffe
Affiliation:
Public Health Laboratory, Level 6/7, John Radcliffc Hospital, Headington, Oxford 0X3 9DU
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Summary

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A comparison was made between membrane filtration and centrifugation for the isolation of Legionella pneumophila from seeded water samples. Using samples of varying concentration, the optimum speed and time of centrifugation were determined and the relationship between the number of organisms present in the water and the proportion recovered was examined. Following this, sequential routine environmental waters were filtered and centrifuged in parallel.

Centrifugation and filtration using nitrocellulose filters were found to be comparable. The optimum speed and time of centrifugation was approximately 6000 g for 10 min. There was a constant proportion of viable organisms recovered irrespective of the concentration in the unspun samples.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 2 , October 1987 , pp. 241 - 247
Copyright
Copyright © Cambridge University Press 1987

References

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