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Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Published online by Cambridge University Press:  15 May 2009

R. W. S. Harvey  and
T. H. Price
R. W. S. Harvey
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff
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Summary

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Selenite F broth and a modified Muller—Kauffmann tetrathionate broth were investigated using sewage-polluted natural water as inocula. The modification of the tetrathionate medium was necessary as commercial alternatives would not allow multiplication of small numbers of salmonellas.

The wide range of molar concentrations in different tetrathionate broths was emphasized.

Selenite F broth was more efficient than Muller—Kauffmann tetrathionate broth, in our hands, with the material tested. Direct inoculation of the enrichment media was used.

Each of the two media examined had a bias towards selection of certain sero types. If possible, both enrichment broths should be used to obtain maximum information.

Type
Research Article
Information
Journal of Hygiene , Volume 77 , Issue 3 , December 1976 , pp. 333 - 339
Copyright
Copyright © Cambridge University Press 1976

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