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Isolates of Salmonella enterica Enteritidis PT4 with enhanced heat and acid tolerance are more virulent in mice and more invasive in chickens

Published online by Cambridge University Press:  15 May 2009

T. J. Humphrey ,
A. Williams ,
K. McAlpine ,
M. S. Lever ,
J. Guard-Petter  and
J. M. Cox
T. J. Humphrey
Affiliation:
PHLS Food Microbiology Research Unit, Church Lane, Heavitree, Exeter, Devon EX2 5ADUK
A. Williams
Affiliation:
Centre for Applied Microbiology & Research, Porton Down, Salisbury, Wiltshire SP4 0JGUK
K. McAlpine
Affiliation:
PHLS Food Microbiology Research Unit, Church Lane, Heavitree, Exeter, Devon EX2 5ADUK
M. S. Lever
Affiliation:
Centre for Applied Microbiology & Research, Porton Down, Salisbury, Wiltshire SP4 0JGUK
J. Guard-Petter
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, 934 College Station Road, Athens, Georgia 30605, USA
J. M. Cox
Affiliation:
Department of Food Science and Technology, University of New South Wales, New South Wales, Sydney 2052, Australia
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Summary

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Two Enteritidis PT4 isolates which differed in inherent tolerance to heat, acid, H2O2 and the ability to survive on surfaces were used to infect mice, day-old chicks or laying hens. The acid-, heat-, H2O2- and surface-tolerant isolate was more virulent in mice and more invasive in laying hens, particularly in reproductive tissue. However, no significant differences were observed in behaviour in chicks. Both PT4 isolates were able to infect chicks housed in the same room as infected birds, although the heat-tolerant isolate survived significantly better than the heat-sensitive one in aerosols.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 1 , August 1996 , pp. 79 - 88
Copyright
Copyright © Cambridge University Press 1996

References

1.Advisory Committee on the Microbiological Safety of Food. Salmonella in eggs. London: HMSO, 1993.Google Scholar
2.Humphrey, TJ, Slater, E, McAlpine, K, Rowbury, RJ, Gilbert, RJ.Salmonella enteritidis PT4 isolates more tolerant of heat, acid, or hydrogen peroxide also survive longer on surfaces. Appl Environ Microbiol 1995; 61: 3161–4.CrossRefGoogle ScholarPubMed
3.Petter, JG.Detection of two smooth colony phenotypes in a Salmonella enteritidis isolate which vary in their ability to contaminate eggs. Appl. Environ Microbiol 1993; 59: 2884–90.CrossRefGoogle Scholar
4.Guard-Petter, J, Lakshmi, B, Carlson, R, Ingram, K.Characterization of lipopolysaccharide heterogeneity in Salmonella enteritidis by an improved gel electro-phoresis method. Appl Environ Microbiol 1995; 61: 2845–51.CrossRefGoogle Scholar
5.Hinton, M, Pearson, GR, Threlfall, EJ, Rowe, B.Experimental Salmonella enteritidis infection in chicks. Vet Rec 1989; 124: 223.CrossRefGoogle ScholarPubMed
6.Hinton, M, Threlfall, EJ, Rowe, B.The invasiveness of different strains of Salmonella enteritidis phage type 4 for young chickens. FEMS Microbiol Lett 1990; 70: 193–6.Google Scholar
7.Hinton, M, Threlfall, E, Rowe, B.The invasive potential of Salmonella enteritidis phage types for young chickens. Lett Appl Microbiol 1990; 10: 237–9.CrossRefGoogle Scholar
8.Cox, JM, Woolcock, JB.Lipopolysaccharide expression and virulence in mice of Australian isolates of Salmonella enteritis. Lett Appl Microbiol 1994; 19: 95–8.CrossRefGoogle Scholar
9.Chart, H, Rowe, B.Antibodies to lipopolysaccharide and outer membrane proteins of Salmonella enteritidis PT4 are not involved in protection from experimental infection. FEMS Microbiol Lett 1991; 84: 345–50.CrossRefGoogle Scholar
10.Baskerville, A, Humphrey, TJ, Fitzgeorge, RB, et al. Airborne infection of laying hens with Salmonella enteritidis phage type 4. Vet Rec 1992; 130: 395–8.CrossRefGoogle ScholarPubMed
11.Humphrey, TJ, Baskerville, A, Chart, H, Rowe, B, Whitehead, A.Infection of laying hens with Salmonella enteritidis PT4 by conjunctival challenge. Vet Rec 1992; 131: 386–8.CrossRefGoogle ScholarPubMed
12.Humphrey, TJ, Whitehead, A, Gawler, AH, Henley, A, Rowe, B.Numbers of Salmonella enteritidis in the contents of naturally contaminated hens' eggs. Epidemiol Infect 1991; 106: 489–96.CrossRefGoogle ScholarPubMed
13.Hambleton, P, Broster, MG, Dennis, PJ, Henstridge, R, Fitzgeorge, R, Conlan, JW.Survival of virulent Legionella pneumophila in aerosols. J Hyg 1983; 90: 451–60.CrossRefGoogle ScholarPubMed
14.Padron, MN.Salmonella typhimurium penetration through the eggshell of hatching eggs. Avian Dis 1990; 34: 463–5.Google ScholarPubMed
15.Gast, RK, Beard, CW.Production of Salmonella enteritidis-contaminated eggs by experimentally infected hens. Avian Dis 1990; 34: 438–46.CrossRefGoogle ScholarPubMed
16.Humphrey, TJ, Baskerville, A, Mawer, S, Rowe, B, Hopper, S.Salmonella enteritidis phage type 4 from the contents of intact eggs: a study involving naturally infected hens. Epidemiol Infect 1989; 103: 415–23.CrossRefGoogle ScholarPubMed
17.Hoop, RK, Pospischil, A.Bacteriological, serological, histological and immuno-histochemical findings in laying hens naturally infected with Salmonella enteritidis phage type 4 infection. Vet Rec 1993; 133: 391–3.CrossRefGoogle Scholar
18.Lister, SA.Salmonella enteritidis infection in broilers and broiler-breeders. Vet Rec 1988; 123: 250.CrossRefGoogle ScholarPubMed
19.Keller, LH, Benson, CE, Krotec, K, Eckroade, RJ.Salmonella enteritidis colonization of the reproductive tract and forming and freshly laid eggs of chickens. Infect Immun 1995; 63: 2443–9.CrossRefGoogle ScholarPubMed
20.Brake, J, Thaxton, P.Physiological changes in caged layers during a forced molt. 2. Gross changes in organs. Poult Sci 1979; 58: 707–16.CrossRefGoogle ScholarPubMed
21.Humphrey, TJ, Baskerville, A, Chart, H, Rowe, B, Whitehead, B.Salmonella enteritidis PT4 infection in specific pathogen free hens: influence of infecting dose. Vet Rec 1991; 129: 482–5.CrossRefGoogle ScholarPubMed
22.Bygrave, A, Gallagher, J.Transmission of Salmonella enteritidis in poultry. Vet Rec 1989; 124: 571.CrossRefGoogle ScholarPubMed
23.Halavatkar, H, Barrow, PL.The role of a 54-kb plasmid in the virulence of strains of Salmonella enteritidis of phage type 4 for chicken and mice. J Med Microbiol 1993; 38: 171–6.CrossRefGoogle Scholar
24.Chart, H, Threlfall, EJ, Rowe, B.Virulence studies of Salmonella enteritidis phage types. Lett Appl Microbiol 1991; 12: 188–91.CrossRefGoogle Scholar
25.Cox, JM.Salmonella enteritidis: virulence factors and invasive infection in poultry. Trends Food Sci Tech 1995; 6: 407–10.CrossRefGoogle Scholar
26.Collinson, SK, Emödy, L, Müller, KH, Trust, TJ, Kay, WW.Purification and characterisation of thin, aggregative fimbriae from Salmonella enteritidis. J. Bacteriol 1991; 173: 4473–81.CrossRefGoogle ScholarPubMed
27.Collinson, SK, Doig, PC, Doran, JL, Clouthier, S, Trust, TJ, Kay, WW.Thin, aggregative fimbriae mediate binding of Salmonella enteritidis to fibrolectin. J Bacteriol 1993; 175: 12–8.CrossRefGoogle Scholar
28.Thorns, CJ, Sojka, MG, Chasey, D.Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using monoclonal antibody. J Clin Microbiol 1990; 28: 2409–14.CrossRefGoogle ScholarPubMed
29.Mekalanos, JJ.Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol 1992; 174: 1–7.CrossRefGoogle ScholarPubMed