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The intestinal flora of colonization-resistant mice

Published online by Cambridge University Press:  15 May 2009

F. Wensinck
Affiliation:
Laboratory of Medical Microbiology, Medical Faculty, Rotterdam, The Netherlands
J. G. H. Ruseler-van Embden
Affiliation:
Laboratory of Medical Microbiology, Medical Faculty, Rotterdam, The Netherlands
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Summary

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To facilitate the isolation of anaerobes from cultures in which swarming organisms were present Roux flasks were adapted to provide anaerobic conditions. Using these flasks, five species of anaerobes were isolated from the caecum of mice resistant to colonization with Escherichia coli and other Gram-negative organisms. These species accounted for the majority of organisms seen in films of caecal material. No aerobes were isolated and the flora of these mice apparently consisted of a limited number of anaerobes representing part of the intestinal flora of conventional mice. Three of the species were clostridia and another was identified as Propionibacterium acnes. One species resembled Catenabacterium but was not identified. One of the Clostridium species could only be isolated when the medium was supplemented with yeast extract and vitamins. This species sporulated on media with ammonium phosphate as the source of nitrogen; tryptone completely suppressed sporulation.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Aranki, A., Syed, S. A., Kenney, E. B. & Freter, R. (1969). Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Applied Microbiology 17, 568.CrossRefGoogle Scholar
Baird-Parker, A. C. (1960). The classification of Fusobacteria from the human mouth. Journal of General Microbiology 22, 458.CrossRefGoogle ScholarPubMed
Barnes, E. M. & Burton, G. C. (1970). The effect of hibernation on the caecal flora of the thirteen-lined ground squirrel (Citellus tridecemlineatus). Journal of Applied Bacteriology 33, 505.CrossRefGoogle ScholarPubMed
Barnes, E. M. & Goldberg, H. S. (1968). The relationship of bacteria within the family Bacteroidaceae as shown by numerical taxonomony. Journal of General Microbiology 51, 313.CrossRefGoogle Scholar
Cruickshank, R. (ed.) (1968). Medical Microbiology, 11th ed.Edinburgh: E. and S. Livingstone Ltd.Google Scholar
Drasar, B. S. (1967). Cultivation of anaerobic intestinal bacteria. Journal of Pathology and Bacteriology 94, 417.CrossRefGoogle ScholarPubMed
Gordon, J. H. & Dubos, R. (1970). The anaerobic bacterial flora of the mouse cecum. Journal of Experimental Medicine 132, 251.CrossRefGoogle ScholarPubMed
Guillaume, J. & Osteux, R. (1955). Détermination des acides aliphatiques volatils de C1 à C6 dans les liquides biologiques par chromatographie sur papier. Comptes Rendus de l' Académie des Sciences (Paris) 241, 501.Google Scholar
Hamilton, R. D. & Zahler, S. A. (1957). A study of Leptotrichia buccalis. Journal of Bacteriology 73, 386.CrossRefGoogle ScholarPubMed
Houte, J. Van & Gibbons, R. J. (1966). Studies of the cultivable flora of normal human feces. Antonie van Leeuwenhoek Journal of Microbiology and Serology 32, 212.CrossRefGoogle ScholarPubMed
Lee, A., Gordon, J. & Dubos, R. (1968). Enumeration of the oxygen sensitive bacteria usually present in the intestine of healthy mice. Nature, London 220, 1137.CrossRefGoogle ScholarPubMed
Long, C. (1947). The stabilization and estimation of lactic acid in blood samples. Biochemical Journal 40, 27.CrossRefGoogle Scholar
Moore, W. E. C. (1966). Techniques for routine culture of fastidious anaerobes. International Journal of Systematic Bacteriology 16, 173.CrossRefGoogle Scholar
Moore, W. E. C., Cato, E. P. & Holdeman, L. V. (1969). Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. Journal of Infectious Diseases, 119, 641.CrossRefGoogle ScholarPubMed
Schaedler, R. W., Dubos, R. & Costello, R. (1965). Association of germfree mice with bacteria isolated from normal mice. Journal of Experimental Medicine 122, 77.CrossRefGoogle ScholarPubMed
Skerman, V. B. D. (1967). A Guide to the Identification of the Genera of Bacteria, 2nd ed.Baltimore: Williams and Wilkins.Google Scholar
Waaij, D. van der (1968). The persistent absence of Enterobacteriaceae from the intestinal flora of mice following antibiotic treatment. Journal of Infectious Diseases 118, 32.CrossRefGoogle ScholarPubMed
Waaij, D. Van der, Berghuis-de Vries, J. M. & Lekkerkerk-van der Wees, J. E. C. (1971). Colonization resistance of the digestive tract in conventional and antibiotic treated mice. Journal of Hygiene 69, 405.CrossRefGoogle ScholarPubMed
Wiel-Korstanje, J. A. A. van der & Winkler, K. C. (1970). Medium for differential count of the anaerobic flora in human feces. Applied Microbiology 20, 168.CrossRefGoogle Scholar