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Interaction of L. pneumophila and a free living amoeba (Acanthamoeba palestinensis)

Published online by Cambridge University Press:  19 October 2009

C. M. Anand ,
A. R. Skinner ,
A. Malic  and
J. B. Kurtz
C. M. Anand
Affiliation:
The Virus Laboratory and Department of Electron Microscopy, John Radcliffe Hospital, Oxford 0X3 9DU
A. R. Skinner
Affiliation:
The Virus Laboratory and Department of Electron Microscopy, John Radcliffe Hospital, Oxford 0X3 9DU
A. Malic
Affiliation:
The Virus Laboratory and Department of Electron Microscopy, John Radcliffe Hospital, Oxford 0X3 9DU
J. B. Kurtz
Affiliation:
The Virus Laboratory and Department of Electron Microscopy, John Radcliffe Hospital, Oxford 0X3 9DU
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Co-cultivation of Legionella pneumophila serogroup I and Acanthamoeba palestinensis in Neff's medium at 35 °C resulted in the intracellular multiplication of the bacteria as demonstrated by electron microscopy and immunofluorescence. In the closed experimental system used, the number of legionellae rose from 107 colony forming units (c. f. u.)/ml initially to a maximum of 1010 c. f. u. /ml on day 5. Legionellae were seen in expelled phagosomes, in some amoebae filling the cytoplasm and in others in which the process of encystment appeared to have commenced.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 2 , October 1983 , pp. 167 - 178
Copyright
Copyright © Cambridge University Press 1983

References

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