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Insertion sequence IS200 fingerprinting of Salmonella typhi: an assessment of epidemiological applicability

Published online by Cambridge University Press:  15 May 2009

E. J. Threlfall ,
E. Torre ,
L. R. Ward ,
A. Dávalos-Pérez ,
B. Rowe  and
I. Gibert
E. J. Threlfall
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
E. Torre
Affiliation:
Departament Patología i Produccions Animals, Facultat de Veterinaria, Universitat Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
L. R. Ward
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
A. Dávalos-Pérez
Affiliation:
Department of Clinical Research, Hospital Vozandes, Quito, Ecuador
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
I. Gibert
Affiliation:
Departament de Genética i Microbiología, Universitad Autónoma de Barcelona 08193 Bellaterra, Barcelona, Spain
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When Pst I–generated digests of genomic DNA from each of the type strains of 49 of the Vi phage types of Salmonella typhi were probed with a PCR–amplified IS200 gene probe, all strains were found to possess at least 11 IS200 elements carried on fragments in the range 24.2–1.2 kb. Fourteen fingerprints were identified but two patterns designated IS200Sty1 and IS200Sty2 predominated. In one strain, a plasmid–mediated IS200 element was identified. When IS200 fingerprinting was applied to epidemiologically–unrelated strains of S. typhi isolated in Ecuador, 3 patterns were identified in 10 strains belonging to 9 different phage types. It is concluded that Vi phage typing remains the method of choice for the primary differentiation of S. typhi but that IS200 fingerprinting may be of limited use in laboratories which do not have access to phage typing.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 2 , April 1994 , pp. 253 - 261
Copyright
Copyright © Cambridge University Press 1994

References

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