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Inhibition of bacterial growth by pure ozone in the presence of nutrients

Published online by Cambridge University Press:  15 May 2009

M. Ingram  and
R. B. Haines
M. Ingram
Affiliation:
From the Low Temperature Station for Research in Biochemistry and Biophysics, University of Cambridge and Department of Scientific and Industrial Research
R. B. Haines
Affiliation:
From the Low Temperature Station for Research in Biochemistry and Biophysics, University of Cambridge and Department of Scientific and Industrial Research
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Data are given showing the concentration of pure ozone required to inhibit the growth of, and to destroy, various micro-organisms when growing on agar, in nutrient broth, a synthetic medium, or simply suspended in water. The following is shown:

1. Different organisms vary in their susceptibility towards this gas. Achromobacter and Pseudomonas strains, such as occur on chilled meat, are the most resistant. On the whole, mould fungi are about as susceptible as bacteria.

2. Very much higher concentrations are required to arrest established growth than can be used if inoculation and admission of inhibitor are coincident.

3. Lower concentrations are inhibitory at lower temperatures. These results are ascribed to dissipation of the ozone by combination with products of bacterial metabolism. It seems that any factor which diminishes growth will augment the effectiveness of ozone.

4. Comparatively small concentrations (less than 10 p.p.m.) suffice to destroy bacteria suspended in water. Somewhat larger concentrations inhibit growth in a synthetic medium.

5. Still larger quantities (several hundred p.p.m.) are required in nutrient broth.

6. Nutrient broth treated with ozone will support little or no bacterial growth, due to change of pH, growth taking place slowly if the pH is restored to its original value. Inhibition of growth in such a medium is therefore a complex process depending in part upon the secondary effects of decomposition of the medium.

7. To kill them, bacteria require still more ozone (in some cases several thousand p.p.m.) if growing on agar. These results are ascribed to combination of ozone with the supporting medium.

8. When applied to organisms growing on food, combination with ozone not only spoils the food, but it makes it difficult to interpret the effect on the microbes in terms of the ozone applied.

9. The inhibitory concentrations are higher than humans can tolerate.

10. Ozone destroys the dehydrogenating enzymes of the cell, and it is suggested that its germicidal action may be partly due to interference with cellular respiration.

The work described in this paper was carried out as part of the programme of the Food Investigation Organization of the Department of Scientific and Industrial Research.

Type
Research Article
Information
Journal of Hygiene , Volume 47 , Issue 2 , June 1949 , pp. 146 - 158
Copyright
Copyright © Cambridge University Press 1949

References

REFERENCES

Anon. (1945). Brit. Chem. Abstr. 39, 2357.Google Scholar
Bancroft, W. D., & Richter, G. H., (1931). The chemistry of disinfection. J. phys. Chem. 35, 511.CrossRefGoogle Scholar
Bergel, F., & Bolz, K., (1933). Über die Autoxydation von Amino-säurederivaten und ihr Abbau durch Ozon. Hoppe-Seyl. Z. 220, 20.CrossRefGoogle Scholar
Cathcart, W. H., Wyberg, R. E., & Merz, A., (1942). The effect of ultra-violet radiation and ozone on bakery custard and cream fillings. Food Res. 7, 1.CrossRefGoogle Scholar
Ewell, A. W., (1938). Present use and future prospects of ozone in food storage. Food Res. 3, 101.CrossRefGoogle Scholar
Ewell, A. W., (1941). Researches on ultra-violet light and ozone. Refrig. Engr. 41, 331.Google Scholar
Griffith, R. O., & Schutt, W. J., (1921). The decomposition of ozone by light of the visible spectrum.. Trans Chem. Soc. 119, 1948–59.CrossRefGoogle Scholar
Haines, R. B., (1937). Microbiology in the Preservation of Animal Tissues p. 68. (Food Investigation Special Report, H.M. Stationery Office.)Google Scholar
Harries, C., & Langheld, K., (1907). Über das Ver-halten der Eiweisspaltprodukte und einiger Zucker-arten gegen Ozon. Hoppe-Seyl. Z. 51, 373.CrossRefGoogle Scholar
Heise, R., (1917a). Über die Einwirkung von Ozon auf Mikroorganismen und kunstliche Nährsubstrate, als Beitrag Zur Kenntniss der Ozonwirkung in Fleisch-kuhlhallen. I. Arb. biol. Abt. (Anst.—Reichsanst.), Berl. 50, 204–31.Google Scholar
Heise, R., (1917b). Über die Einwirkung von Ozon auf Mikroorganismen und künstliche Nährsubstrate, als Beitrag Zur Kenntniss der Ozonwirkung in Fleisch-kuhlhallen. II. Arb. biol. Abt. (Anst.üReichsanst.), Berl. 50, 419.Google Scholar
Hill, L., & Flack, M., (1911). The physiological influence of ozone. Proc. Roy. Soc. B, 84, 404.Google Scholar
Käss, G., (1936). Einfluss von Ozon auf Haltbarkeit von Fleisch. Z. des. Kälteindustr. 43, 152.Google Scholar
Kefford, J. F., (1948). Effect of Ozone on Microbial Growth in Beef Muscle and on the Flavour of Beef Fat. J. Coun. sci. Industr. Res. Aust. 21, 116.Google Scholar
Keilin, D., (1928). Cytochrome and respiratory enzymes. Proc. Roy. Soc. B, 104, 206.Google Scholar
Macara, T. J. R., (1947). Removal of taint from frozen meat and other foodstuffs. Mod. Refrig. 50, 63.Google Scholar
Mellor, J., (1922). A Comprehensive Treatise in Inorganic and Theoretical Chemistry. Vol. I.CrossRefGoogle Scholar
Nelson, C. I., (1926). Growth studies on B. coli. J. Infect. Dis. 38, 371.CrossRefGoogle Scholar
Quastel, J. H., & Wooldridge, W. R., (1927). The effects of chemical and physical changes in environment on resting bacteria. Biochem. J. 21, 148.CrossRefGoogle ScholarPubMed
Strecker, W., & Thienemann, H., (1920). Über die Einwirkung von Ozon auf die Alkalimetalle, Am-moniak, and Substitutions Produkte des Ammoniaks. Ber. dtsch. chem. Ges. 53, 2096.CrossRefGoogle Scholar
Taylor, J. M., (1947). Some characteristics of ozone in relation to water treatment. J. Inst. Water Eng. 1, 187.Google Scholar
Tomkins, R. G., (1932). The action of certain volatile substances and gases on the growth of mould fungi. Proc. Roy. Soc. B, 111, 210.Google Scholar
Treadwell, F. P., & Anneler, E., (1905). Zur quanti-tativen Bestimmung des Ozons. Z. anorg. Chem. 48, 86.CrossRefGoogle Scholar