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The influence of technique on the sensitivity of monkeys to intraspinally inoculated poliovirus

Published online by Cambridge University Press:  15 May 2009

T. S. L. Beswick  and
C. R. Coid
T. S. L. Beswick
Affiliation:
Biological Standards Control Laboratory, Medical Research Council Laboratories, London, N.W.3
C. R. Coid
Affiliation:
Biological Standards Control Laboratory, Medical Research Council Laboratories, London, N.W.3
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1. Two methods of injecting monkeys intraspinally are compared.

2. It has been shown that the technique of inserting the needle through the cord and withdrawing it 1–2 mm. before injection (Sabin's technique) gives a less widespread distribution of inoculum than the alternative method of making the injection as soon as the tip of the needle is within the substance of the spinal cord (‘standard technique’).

3. When attenuated Type 3 poliovirus is injected into cynomolgus monkeys by Sabin's technique, the incidence of paralysis and poliomyelitis lesions observed is less than when it is injected by the ‘standard technique’.

4. The relative merits of the two techniques are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 59 , Issue 4 , December 1961 , pp. 395 - 399
Copyright
Copyright © Cambridge University Press 1961

References

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