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The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests

Published online by Cambridge University Press:  15 May 2009

Janet M. Bradbury  and
F. T. W. Jordan
Janet M. Bradbury
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station, ‘Leahurst’, Neston, Wirral, Cheshire L64 7TE
F. T. W. Jordan
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station, ‘Leahurst’, Neston, Wirral, Cheshire L64 7TE
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Mycoplasma gallisepticum antigens were prepared from organisms cultured in broth medium with glucose. The influence of period of growth, pH of the medium and duration of incubation at low pH (5·0) on the sensitivity of these antigens was determined in certain tests. The most sensitive antigen for the serum plate test was harvested after no more than 8 hr. incubation at pH 5·0. Sensitivity in serum plate, haemagglutination and gel diffusion tests was impaired if organisms were incubated at pH 5·0 for long periods. Antigens prepared from buffered broth medium were found to be at least as sensitive as those from unbuffered medium for the haemagglutination and gel precipitation tests, but considerably less so for the serum plate test.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 4 , December 1971 , pp. 593 - 606
Copyright
Copyright © Cambridge University Press 1971

References

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