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Incrimination of an environmental source of a case of Legionnaires' disease by pyrolysis mass spectrometry

Published online by Cambridge University Press:  15 May 2009

P. R. Sisson ,
R. Freeman ,
N. F. Lightfoot  and
I. R. Richardson
P. R. Sisson
Affiliation:
Regional Public Health Laboratory, Newcastle upon Tyne
R. Freeman*
Affiliation:
Department of Microbiology, University of Newcastle upon Tyne
N. F. Lightfoot
Affiliation:
Regional Public Health Laboratory, Newcastle upon Tyne
I. R. Richardson
Affiliation:
Regional Public Health Laboratory, Newcastle upon Tyne
*
*Dr. R. Freeman, Department of Microbiology, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne.
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Summary

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Following a case of Legionnaires' disease Legionella pneumophila of the same serogroup was isolated from the hot water system of the hotel which had been epidemiologically implicated as the source of the infection. Pyrolysis mass spectrometry (PyMS) was used to compare 11 isolates from the hotel water sample with the patient's strain. Epidemiologically unrelated isolates, both clinical and environmental, of the same serogroup and monoclonal antibody type of L. pneumophila were included in the same analysis, together with relevant reference strains. The patient strain was shown to be indistinguishable from seven of the hotel water isolates, but clearly different from other unrelated clinical isolates, the reference strains and some of the other environmental isolates. PyMS is a rapid and cheap method for inter-strain comparison for L. pneumophila.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 1 , August 1991 , pp. 127 - 132
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Joly, JR, McKinney, RM, Tobin, JO, Bibb, WF, Watkins, ID, Ramsay, D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol 1986; 23: 768–71.CrossRefGoogle ScholarPubMed
2.Saunders, NA, Harrison, TG, Haththotuwa, A, Kachwalla, N, Taylor, AG. A method for typing strains of Legionella pneumophila serogroup 1 by analysis of restriction fragment length polymorphisms. J Med Microbiol 1990; 31: 45–55.CrossRefGoogle ScholarPubMed
3.Kajioka, R, Tang, PW. Curie-point pyrolysis mass-spectrometry of Legionella species. J Anal Appl Pyrolysis 1984; 6: 59–60.CrossRefGoogle Scholar
4.Bopp, CE, Sumner, JW, Morris, GK, Wells, JE. Isolation of Legionella spp. From environmental water samples by low pH treatment and use of a selective medium. J Clin Microbiol 1981; 13: 614–719.CrossRefGoogle Scholar
5.Dennis, PJ, Green, D, Jones BPC. A note on the temperature tolerance of Legionella. J Appl Bacteriol 1984; 56: 349–50.CrossRefGoogle Scholar
6.Aries, RE, Gutteridge, CS, Ottley, TW. Evaluation of a low-cost automated pyrolysis mass spectrometer. J Anal Appl Pyrolysis 1986; 9: 81–98.CrossRefGoogle Scholar
7.Freeman, R, Goodfellow, M, Gould, FK, Hudson, SJ, Lightfoot, NF. Pyrolysis-mass spectrometry (Py-MS) for the rapid epidemiological typing of clinically significant bacterial pathogens. J Med Microbiol 1990; 32: 283–6.CrossRefGoogle ScholarPubMed
8.Freeman, R, Gould, FK, Wilkinson, R, Ward, AC, Lightfoot, NF, Sisson, PR. Rapid inter-strain comparison by pyrolysis mass spectrometry of coagulase negative staphylococci from persistent CAPD peritonitis. Epidemiol Infect. In press.Google Scholar
9.Harrison, TG, Saunders, NA, Haththotuwa, A, Hallas, G, Birtles, RJ, Taylor, AG. Phenotypic variation amongst genotypically homogeneous Legionella pneumophila serogroup 1 isolates: implications for the investigation of outbreaks of Legionnaires' disease. Epidemiol Infect 1990; 104: 171–80.CrossRefGoogle ScholarPubMed
10.Colbourne, JS, Dennis, PJ, Trew, RM, Berry, C, Vesey, G. Legionella and public water supplies. Water Sci Technol 1988; 20: 5–10.CrossRefGoogle Scholar
11.Magee, JT, Hindmarch, JM, Burnett, IA, Pease, A. Epidemiological typing of Streptococcus pyogenes by pyrolysis mass spectrometry.J Med Microbiol 1989; 30: 273–8.CrossRefGoogle ScholarPubMed