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The incidence of virulence factors in mesophilic Aeromonas species isolated from farm animals and their environment

Published online by Cambridge University Press:  15 May 2009

S. J. Gray ,
D. J. Stickler  and
T. N. Bryant
S. J. Gray
Affiliation:
Public Health Laboratory Service, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
D. J. Stickler
Affiliation:
School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 13, Cardiff CF1 3XF
T. N. Bryant
Affiliation:
Medical Statistics and Computing, University of Southampton, South Academic Block, Southampton General Hospital, Southampton S09 4XY
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Sixty-one isolates of Aeromonas spp. from the faeces of pigs, cows and a variety of associated environmental sources were examined for the characteristics that are reputed to have roles in pathogenicity. Most isolates of Aeromonas hydrophila were cytotoxic (96·4%) and were capable of producing cell elongation factor (75%) and haemagglutinins (67·9%). In contrast few of the Aeromonas caviae isolates produced these three markers (13·6 %, 27·3% and 36·4% respectively). In general, Aeromonas sobria occupied an intermediate position (36·4%, 27·3% and 54·5%), but they did produce the highest mean invasion index for HEp-2 cells. Statistical analysis revealed significant associations between the carriage of these factors and it was clear that many isolates of aeromonads from water and animals possessed the full battery of putative virulence factors.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 2 , October 1990 , pp. 277 - 294
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Buchanan, RL, Palumbo, SA. Aeromonas hydrophila and Aeromonas sobria as potential food poisoning species: A review. J Food Safety 1985; 7: 15–29.Google Scholar
2.Atkinson, HM, Trust, TJ. Haemagglutination properties and adherance ability of Aeromonas hydrophila. Infect Immun 1980; 27: 938–46.CrossRefGoogle ScholarPubMed
3.Daily, OP, Joseph, SW, Coolbaugh, JC et al. , Association of Aeromonas sobria with human infection. J Clin Microbiol 1981; 13: 769–77.CrossRefGoogle ScholarPubMed
4.Jiwa, SFH. Enterotoxigenicity, haemagglutination and cell-surface hydrophobicitv in Aeromonas hydrophila, A. sobria and A; salmonicida. Vet Microbiol 1983; 8: 17–34.CrossRefGoogle ScholarPubMed
5.Sanyal, SC, Singh, SJ, Sen, PC. Enteropathogenicity of Aeromonas ydrophila and Plesiomonas shigelloides. J Med Microbiol 1975; 8: 195–8.CrossRefGoogle ScholarPubMed
6.Ljungh, A, Popoff, M, Wadström, T. Aeromonas hydrophila in acute diarrhoeal disease: Detection of enterotoxin and biotyping of strains. J Clin Microbiol 1977; 6: 96–100.CrossRefGoogle ScholarPubMed
7.Bundell, CS, Watson, IM, Burke, V, Gracey, M. Protection of rats against cholera toxin and cholera-like enterotoxins by immunization with enteric-coated cholera toxin. Ann Trop Paediatr 1986; 6: 199–204.CrossRefGoogle ScholarPubMed
8.Chopra, AK, Houston, CW, Genaux, CT, Dixon, JD, Kurosky, A. Evidence for production of an enterotoxin and cholera toxin cross-reactive factor by Aeromonas hydrophila. J Clin Microbiol 1986; 24: 661–4.CrossRefGoogle ScholarPubMed
9.Asao, T, Kinoshita, Y, Kozaki, S, Uemura, T, Sakaguchi, G. Purification and some properties of Aeromonas hydrophila haemolysin. Infect Immun 1984; 46: 122–7.CrossRefGoogle Scholar
10.Notermans, S, Havelaar, A, Jansen, W, Kozaki, S, Guinée, P. Production of ‘Asao Toxin ’ by Aeromonas strains isolated from faeces and drinking water. J Clin Microbiol 1986; 23: 1140–2.Google Scholar
11.Watson, IM, Robinson, JO, Burke, V, Gracey, M. Invasiveness of Aeromonas spp. in relation to biotype, virulence factors, and clinical features. J Clin Microbiol 1985; 22: 48–51.CrossRefGoogle ScholarPubMed
12.Lawson, MA, Burke, V, Chang, BJ. Invasion of HEp–2 cells by faecal isolates of Aeromonas hydrophila. Infect Immun 1985; 47: 680–3.CrossRefGoogle ScholarPubMed
13.Gray, SJ, Stickler, DJ. Some observations on the faecal carriage of mesophilic Aeromonas species in cows and pigs. Epidemiol Infect 1989; 103: 523–37.CrossRefGoogle ScholarPubMed
14.Chakraborty, T, Montenegro, MA, Sanyal, SC, Helmuth, R, Bulling, E, Timmis, KN. Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin. Infect Immun 1984; 46: 435–41.CrossRefGoogle ScholarPubMed
15.Giugliano, LG, Mann, GF, Draser, BS. Response of mammalian cell lines to the toxins of Escherichia coli. J Med Microbiol 1982; 15: 531–9.CrossRefGoogle Scholar
16.Miles, AA, Misra, SS. The estimation of the lactericidal power of the blood. J Hyg 1938; 38: 732–48.Google ScholarPubMed
17.Burke, V, Cooper, M, Robinson, J et al. , Haemagglutination patterns of Aeromonas spp. in relation to biotype and source. J Clin Microbiol 1984; 19: 39–43.CrossRefGoogle ScholarPubMed
18.Ishigura, EE, Ainsworth, T, Trust, TJ, Kay, WW. Congo red agar, a differential medium for Aeromonas salmonicida, detects the presence of the cell surface protein array involved in virulence. J Bact 1985; 164: 1233–7.CrossRefGoogle Scholar
19.Gray, SJ. Aeromonas hydrophila in livestock; incidence, biochemical characteristics and antibiotic susceptibility. J Hyg 1984; 92: 365–75.Google Scholar
20.Lee, JV, Donovan, TJ, Vibrio, Aeromonas, and Plesiomonas. In: Collins, CH, range, JH, eds. Isolation and identification of micro-organisms of medical and veterinary importance. Society for Applied Bacteriology, London: Academic Press, 1985: 13–33.Google Scholar
21.Scharmann, W. Vorkommen von Elastase bei Pseudomonas und Aeromonas. Zentralbl Bakteriol Mikrobiol Hyg (A) 1972; 220: 435–42.Google Scholar
22.Marandon, J-L, Oeding, P. Investigations on animal Staphylococcus aureus strains. I. Biochemical characteristics and phage-typing. Acta Pathol Microbiol Scand 1966; 67: 149–56.Google Scholar
23.Gray, SJ. Characterisation of Aeromonas sp. in the API ZYM system. Med Lab Sci 1987; 44: 287–9.Google Scholar
24.Burke, V, Robinson, J, Gracey, M, Peterson, D, Partridge, K. The isolation of A. hydrophila from a metropolitan water supply: Seasonal correlation with clinical isolates. Appl Environ Microbiol 1984; 48: 361–6.CrossRefGoogle ScholarPubMed
25.Rahim, Z, Sanyal, SC, Aziz, KMS, Huq, MI, Chowdhury, AA. Isolation of enterotoxigenic, haemolytic and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh. Appl Environ Microbiol 1984; 48: 865–7.CrossRefGoogle ScholarPubMed
26.Figura, N, Marri, L. Isolation of Aeromonas species from animals. Eur J Clin Microbiol 1985: 4: 354–55.Google Scholar
27.Callister, SM, Agger, VVA. Enumeration and characterization of Aeromonas hydrophila and Aeromonas caviae isolated from grocery store produce. Appl Environ Microbiol 1987; 53: 249–53.Google Scholar
28.Jablonska-Strynkowska, W, Potapa, M. Aeromonas hydrophila var. anaerogenes as a supposed aetiological agent of food poisoning. Przegl Epidemiol 1983; 37: 455–8.Google Scholar
29.Flynn, TJ, Knepp, IG. Seafood shucking as an etiology for Aeromonas hydrophila infection. Arch Intern Med 1987; 147: 1816–7.Google Scholar
30.Cumberbatch, N, Gurwith, MJ, Langston, C, Sack, RB, Brunton, JL. Cytotoxic enterotoxin produced by Aeromonas hydrophila: Relationship of toxigenic isolates to diarrhoeal disease. Infect Immun 1979; 23: 829–37.Google Scholar
31.Burke, V, Robinson, J, Atkinson, HM, Gracey, M. Biochemical characteristics of entero toxigenic Aeromonas spp. J Clin Microbiol 1982; 15: 48–52.CrossRefGoogle Scholar
32.Donta, ST, Haddow, AD. Cytotoxic activity of Aeromonas hydrophila. Infect Immun 1978; 21: 989–93.CrossRefGoogle ScholarPubMed
33.Figura, N, Marri, L, Verdiani, S, Ceccherini, C, Barberi, A. Prevalence, species differentiation and toxigenicitv of Aeromonas strains in cases of childhood gastroenteritis and in controls. J Clin Microbiol 1986; 23: 595–9.Google Scholar
34.Barer, MR, Millership, SE, Tabaqchali, S. Relationship of toxin production to species in the genus Aeromonas. J Med Microbiol 1986; 22: 303–9.CrossRefGoogle ScholarPubMed
35.Turnbull, PCB, Lee, JV, Miliotis, MD et al. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol 1984; 19: 175–80.CrossRefGoogle ScholarPubMed
36.Adams, D, Atkinson, HM, Woods, WH. Aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes and yeast cells. J Clin Microbiol 1983: 17: 422–7.CrossRefGoogle ScholarPubMed
37.Burke, V, Cooper, M, Robinson, J. Haemagglutination patterns of Aeromonas spp. related to species and source of strains. Aust J Exp Biol Med Sci 1986; 64: 563–70.Google Scholar
38.Statner, B, George, WL. Congo red uptake by motile Aeromonas species. J Clin Microbiol 1987; 25: 876–8.Google Scholar
39.Bunning, VK, Crawford, RG, Stelma, GN, Kaylor, LO, Johnson, CH. Melanogenesis in murine B16 cells exposed to Aeromonas hydrophila cytotoxic enterotoxin. Can J Microbiol 1986; 32: 814–19.Google Scholar
40.Chakraborty, T, Huhle, B, Hof, H, Bergbauer, H, Goebel, W. Marker exchange mutagenesis of the aerolysin determinant in Aeromonas hydrophila demonstrates the role of aerolysin in A. hydrophila-associated systemic infections. Infect Immun 1987: 55: 2274–80.Google Scholar
41.Okitsu, T, Asai, Y, Yasuda, T, Takizawa, K. Studies of Aeromonas spp. isolated from patients with sporadic diarrhoea. Kansenshogaku Zasshi 1985; 59: 977–83.CrossRefGoogle Scholar
42.Ljungh, A, Wadström, T. Aeromonas toxins. Pharmacol Ther 1982; 15: 339–54.Google Scholar
43.Dubey, RS, Sanyal, SC. Enterotoxicity of Aeromonas hydrophila: skin responses and in vivo neutralization. Zentralbl Bakteriol Mikrobiol Hyg (A) 1978; 242: 487–99.Google Scholar
44.Dubey, RS, Sanyal, SC. Characterisation and neutralisation of Aeromonas hydrophila enterotoxin in the rabbit ileal-loop model. J Med Microbiol 1979; 12: 347–54.CrossRefGoogle ScholarPubMed
45.Fehlhaber, K, Scheibner, G. Untersuchungen über Eigenschaften von Aeromonas hydrophila – enterotoxinen aus lebensmittel – hygienischer Sicht. Arch Exp Vet Med 1987; 41: 121–6.Google Scholar
46.O'Reilly, T, Day, DF. Effects of cultural conditions on protease production by Aeromonas hydrophila. Appl Environ Microbiol 1983; 45: 1132–5.Google Scholar
47.Mellergaard, S. Purification and characterization of a new proteolytic enzyme produced by Aeromonas salmonicida. J Appl Bact 1983; 54: 289–94.CrossRefGoogle ScholarPubMed
48.Merkel, JR, Traganza, ED, Mukherjee, BB, Griffin, TB, Prescott, JM. Proteolytic activity and general characteristics of a marine bacterium, Aeromonas proteolytica sp. J Bact 1964; 87: 1227–33.CrossRefGoogle ScholarPubMed
49.Atkinson, HM, Adams, D, Savvas, RS, Trust, TJ. Aeromonas adhesin antigens. In: Research on Aeromonas and Plesiomonas. Experientia 1987; 43: 372–3.Google Scholar
50.Sanyal, SC, Agarwal, RK, Annapurna, E. Haemagglutination properties and fimbriation in enterotoxigenic Aeromonas hydrophila strains. Ind J Med Res 1975; 78: 324–30.Google Scholar
51.Pitarangsi, C, Echeverria, P, Whitmire, R et al. , Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigdloides: Prevalence among individuals with and without diarrhoea in Thailand. Infect Immun 1982; 35: 666–73.Google Scholar
52.Janda, JM, Brenden, R. Importance of Aeromonas sobria in Aeromonas bacteraemia. J Infect Dis 1987; 155: 589–91.CrossRefGoogle Scholar