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Incidence and properties of Staphylococcus aureus associated with turkeys during processing and further-processing operations

Published online by Cambridge University Press:  19 October 2009

B. W. Adams  and
G. C. Mead
B. W. Adams
Affiliation:
Agricultural Research Council Food Research Institute, Colney Lane, Norwich, NR4 7UA, U.K.
G. C. Mead
Affiliation:
Agricultural Research Council Food Research Institute, Colney Lane, Norwich, NR4 7UA, U.K.
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The incidence of Staphylococcus aureus on turkeys sampled at various stages of processing and further-processing was determined on four occasions at each of three different processing plants. For freshly-slaughtered birds, counts from neck skin varied from plant to plant over the range < 102 to > 105/g but in all cases the corresponding counts obtained from carcasses sampled after chilling rarely exceeded 103/g and the same was true for samples of mechanically recovered meat (MRM), the final raw product examined.

Despite the limited susceptibility of isolates from the different factories to typing by means of either standard human or poultry bacteriophages (55–94% untypable), evidence was obtained with the aid of biotyping for the presence of both human and animal-derived strains. However, some biotypes isolated from MRM were not detected at earlier stages of processing.

At one processing plant, an ‘indigenous’ type of S. aureus was clearly demonstrated. It occurred in high numbers in the defeathering machines (up to 105/swab), was found on carcasses at all subsequent stages of processing over the survey period and was shown to survive routine cleaning and disinfection procedures. Isolates of this type produced unusually large amounts of extracellular ‘slime’ in artificial culture.

Two of the three processing plants yielded isolates which were enterotoxigenic. Of 55 strains from Plant 1, 60% produced enterotoxin C and all were of the ‘indigenous’ type. In the case of Plant 2, only two type D-and one type F-producing strain were found.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 3 , December 1983 , pp. 479 - 490
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Blair, J. E. & Williams, R. E. O. (1961). Phage typing of staphylococci. Bulletin of the World Health Organization 24, 771–784.Google Scholar
Christie, R. & Wilson, H. (1941). A test of staphylococcal fibrinolysis. Australian Journal of Experimental Biology and Medical Science 19, 329–332.Google Scholar
Devriese, L. A. (1980). Pathogenic staphylococci in poultry. World's Poultry Science Journal 36, 227–230.Google Scholar
Devriese, L. A., Devos, A. H. & van Damme, L. R. (1975). Quantitative aspects of the Staphylococcus aureus flora of poultry. Poultry Science 54, 95–101.Google Scholar
Donnelly, C. B., Leslie, J. E., Black, L. A. & Lewis, K. H. (1967). Serological identification of enterotoxigenic staphylococci from cheese. Applied Microbiology 15, 1382–1387.Google Scholar
Gibbs, P. A., Patterson, J. T. & Harvey, J. (1978). Biochemical characteristics and enterotoxigenicity of Staphylococcus aureus strains isolated from poultry. Journal of Applied Bacteriology 44, 57–74.Google Scholar
Gibbs, P. A., Patterson, J. T. & Thompson, J. K. (1978 a). Characterization of poultry isolates of Staphylococcus aureus by a new set of poultry phages. Journal of Applied Bacteriology 44, 387–400.Google Scholar
Gibbs, P. A., Patterson, J. T. & Thompson, J. K. (1978 b). The distribution of Staphylococcus aureus in a poultry processing plant. Journal of Applied Bacteriology 44, 401–410.Google Scholar
Hájek, V. & Maršálek, E. (1971). The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abt. Orig. 1A 217, 176–182.Google Scholar
Hájek, V. & Maršálek, E. (1973). The occurrence of enterotoxigenic Staphylococcus aureus strains in hosts of different animal species. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abt. Orig. 1A 223, 63–68.Google Scholar
Harvey, J., Patterson, J. T. & Gibbs, P. A. (1982). Enterotoxigenicity of Staphylococcus aureus strains isolated from poultry: raw poultry carcases as a potential food poisoning hazard. Journal of Applied Bacteriology 52, 251–258.Google Scholar
Jeffries, C. E., Holtman, D. F. & Guse, D. G. (1957). Rapid method for determining the activity of microorganisms on nucleic acids. Journal of Bacteriology 73, 590–591.Google Scholar
Lacey, R. W. (1980). Rarity of gene transfer between animal and human isolates of Staphylococcus aureus in vitro. Journal of General Microbiology 119, 437–442.Google Scholar
Lachica, R. V. F., Genigeorgis, C. & Hoeprich, P. D. (1971). Metachromatic agar diffusion methods for detecting staphylococcal nuclease activity. Applied Microbiology 21, 585–587.Google Scholar
Lahellec, C., Colin, P. & Meurier, C. (1977). Origine et charactérisation des souches de Staphylococcus aureus présentes sur les carcasses de volailles et dans les produits transformés. Bulletin d'Information, Station Experimeniale d'Aviculture de Ploufragan 17, 84–98.Google Scholar
Martley, F. G., Jarvis, A. W., Bacon, D. F. & Lawrence, R. C. (1970). Typing of coagulase positive staphylococci by proteolytic activity on buffered caseinate agar, with special reference to bacteriophage non-typable strains. Infection and Immunity 2, 439–442.Google Scholar
Martley, F. G., Jayashankar, S. R. & Lawrence, R. C. (1970). An improved agar medium for the detection of proteolytic organisms in total bacterial counts. Journal of Applied Bacteriology 33, 363–370.Google Scholar
Mead, G. C. & Thomas, N. L. (1973). Factors affecting the use of chlorine in the spin chilling of eviscerated poultry. British Poultry Science 14, 99–117.Google Scholar
Meyer, W. (1967). Über die Brauchbarkeit des Kristallviolett – Testes zur Differenzibrung von Staphylococcus aureus – Stämmen. Zeitschrift für Medizinische, Mikrobiologie und Immunologie 153, 158–168.Google Scholar
Notermans, S., Firstenbero-Eden, R. & Van Schothorst, M. (1979). Attachment of bacteria to teats of cows. Journal of Food Protection 42, 228–232.Google Scholar
Notermans, S., Dufrenne, J. & Van Leeuwen, W. J. (1982). Contamination of broiler chickens by Staphylococcus aureus during processing: incidence and origin. Journal of Applied Bacteriology 52, 275–280.Google Scholar
Parker, M. T. (1972). Phage typing of Staphylococcus aureus. in Methods in Microbiology, Vol. 7B (ed. Norris, J. R. and Ribbons, D. W.), pp. 2–28. London and New York: Academic Press.Google Scholar
Robbins, R., Gould, S. & Bergdoll, M. (1974). Detecting the enterotoxigenicity of Staphylococcus aureus strains. Applied Microbiology 28, 946–950.Google Scholar
Shimizu, A. (1977). Establishment of a new bacteriophage set for typing avian staphylococci. American Journal of Veterinary Research 38, 1601–1605.Google Scholar
Shiozawa, K., Kato, E. & Shimizu, A. (1980). Enterotoxigenicity of Staphylococcus aureus strains isolated from chickens. Journal of Food Protection 43, 683–685.Google Scholar
Smith, B. A. & Baird-Parker, A. C. (1964). The use of sulphamezathine for inhibiting Proteus spp. on Baird-Parker's isolation medium for Staphylococcus aureus. Journal of Applied Bacteriology 27, 78–82.Google Scholar
Smith, W. W., James, G. A., Miner, M. L., Blommer, E. & Jensen, M. L. (1961 a). A phage typing system for staphylococci from turkeys with synovitis. American Journal of Veterinary Research 22, 388–390.Google Scholar
Smith, W. W., Miner, M. L., Thomas, J. A., Carter, P. B. & Elsner, Y. Y. (1961 b). Evidence of late transmission of Staphylococcus aureus strains within a divided flock of turkeys after 11 weeks of age. American Journal of Veterinary Research 22, 753–756.Google Scholar
Straka, R. P. & Stokes, J. L. (1957). Rapid destruction of bacteria in commonly used diluents and its elimination. Applied Microbiology 5, 21–25.Google Scholar
Thompson, J. K., Gibbs, P. A. & Patterson, J. T. (1980). Staphylococcus aureus in commercial laying flocks: incidence and characteristics of strains isolated from chicks, pullets and hens in an integrated commercial enterprise. British Poultry Science 21, 315–330.Google Scholar
Wilkinson, B. J. & Holmes, K. M. (1979). Staphylococcus aureus cell surface: capsule as a barrier of bacteriophage adsorption. Infection and Immunity 23, 549–552.Google Scholar