Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-22T15:38:50.651Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo transfer of an R factor within the lower gastro-intestinal tract of sheep

Published online by Cambridge University Press:  15 May 2009

M. G. Smith
M. G. Smith
Affiliation:
CSIRO, Division of Food Research, Meat Research Laboratory, P.O. Box 12, Cannon Hill, Queensland, Australia4170
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The transfer of an R factor from donor E. coli introduced into the rumen of adult sheep to strains of the coliform microflora resident post rumen in the lower gastro-intestinal tract was found to be greatly increased when the animals were subjected to a short period of starvation (ca. 24–48 h). This also resulted in coliform organisms containing the resistance determinants of the R factor being excreted for much longer periods, sometimes for months afterwards. As no antibiotic treatment was given to the animals during these experiments, possession of the R factor should have conferred no selective advantages on the host cells and other plasmids could possibly be transferred similarly in vivo in sheep or other ruminants and perhaps also within the gut of monogastric animals.

Type
Research Article
Information
Journal of Hygiene , Volume 79 , Issue 2 , October 1977 , pp. 259 - 268
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Anderson, E. S. (1975). Viability of, and transfer of a plasmid from, E. coli K12 in the human intestine. Nature, London 225, 502–4.CrossRefGoogle Scholar
Brinton, C. C. (1965). The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram-negative bacteria. Transactions of the New York Academy of Sciences 27, 1003–54.CrossRefGoogle Scholar
Cooke, E. Mary, Hettiaratchy, Ira G. T. & Buck, A. C. (1972). Fate of ingested Escherichia coli in normal persons. Journal of Medical Microbiology 5, 361–9.Google Scholar
Hayes, W. (1957). The kinetics of the mating process in Escherichia coli. Journal of General Microbiology 16, 97–119.CrossRefGoogle ScholarPubMed
Lin, E. C. C., Lerner, S. A., & Sally, Jorgensen E. (1962). A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes. Biochimica et biophysica acta 60, 422–4.Google Scholar
Robinet, , Harriette, G. (1962). Relationships of host antibody to fluctuations in Escherichia coli serotypes in the human intestine. Journal of Bacteriology 84, 896–901.Google Scholar
Savage, D. C., Dubos, B. & Schaedler, R. W. (1968). The gastrointestinal epithelium and its autochthonous bacterial flora. Journal of Experimental Medicine 127, 67–75.CrossRefGoogle ScholarPubMed
Sears, H. J., Janes, , Helen, , Saloum, R., Brownlee, Inez & Lamoreaux, L. F., (1956). Persistence of individual strains of Escherichia coli in man and dog under varying conditions. Journal of Bacteriology 71, 370–2.CrossRefGoogle ScholarPubMed
Smith, H. Williams (1966). The incidence of infective drug resistance in strains of Escherichia coli isolated from diseased human beings and domestic animals. Journal of Hygiene 64, 465–74.Google ScholarPubMed
Smith, M. G. (1975). In vivo transfer of R factors between Escherichia coli strains inoculated into the rumen of sheep. Journal of Hygiene 75, 363–70.Google Scholar
Smith, M. G. (1977). Transfer of R factors from Escherichia coli to salmonellas in the rumen of sheep. Journal of Medical Microbiology 10, 29–35.CrossRefGoogle ScholarPubMed
Watanabe, T. & Fukasawa, T. (1961). Episome-mediated transfer of drug resistance in Enterobacteriaceae. 1. Transfer of resistance factors by conjugation. Journal of Bacteriology 81, 669–78.CrossRefGoogle Scholar