Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-19T12:44:23.520Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo and in vitro studies on temperature-sensitive mutants of swine vesicular disease virus

Published online by Cambridge University Press:  15 May 2009

K. J. Preston  and
A. J. M. Garland
K. J. Preston
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
A. J. M. Garland
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two temperature-sensitive mutants of the Ukg 27/72 strain of swine vesicular disease virus were isolated in tissue culture and a third was derived following adaptation in mice. All three were found to have similar growth restrictive temperatures, but varied considerably in their virulence when administered to pigs. The route of inoculation appeared to exert a considerable influence on the apparent degree of attenuation, the antibody titre engendered and the transmission of disease to pigs held in contact with inoculated animals. One strain appeared almost totally attenuated when inoculated into pigs but spread to animals in contact causing severe disease. Virus re-isolated from one such animal was found to have retained its temperature sensitive phenotype, suggesting that virulence in this case was not directly related to temperature sensitivity. Pigs with high antibody titres were found to be susceptible when placed in contact with challenge animals, although the lesions which developed were mild.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 319 - 330
Copyright
Copyright © Cambridge University Press 1979

References

Burrows, R., Mann, J. A. & Goodridge, D. (1974). Swine vesicular disease: comparative studies of viruses isolated from different countries. Journal of Hygiene 73, 109–17.CrossRefGoogle ScholarPubMed
Cooper, P. D. (1961). An improved agar-cell suspension plaque assay for polio virus: some factors affecting efficiency of plating. Virology 13, 153–7.CrossRefGoogle Scholar
Cooper, P. D. (1964). The mutation of poliovirus by 5-fluorouracil. Virology 22, 186–92.CrossRefGoogle ScholarPubMed
De Castro, M. P. (1964). Behaviour of the foot-and-mouth disease virus in cell cultures: susceptibility of the IB-RS-2 cell line. Archivos de Instituto de Biologia, Sao Paulo 31, 63–78.Google Scholar
Fenner, F. (1968 a). In The Biology of Animal Viruses, pp. 706–7. New York: Academic Press.Google Scholar
Fenner, F. (1968 b). In Viral and Rickettsial Infections of Animals(ed. Betts, A. O. and York, C. J.). New York: Academic Press.Google Scholar
Fields, B. N. & Raine, G. S. (1972). Altered disease in rats due to reovirus type 3. Journal of Clinical Investigation 51, 30a.Google Scholar
Flamand, A. (1970). Etude génétique du virus de la stomatite vésiculaire: classement de mutants thermosensibles spontanés en groupes de complémentation. Journal of General Virology 8, 187–95.CrossRefGoogle Scholar
Golding, Susan M., Hedger, R. S., Talbot, P. & Watson, J. (1976). Radial immuno-diffusion and serum neutralisation techniques for the assay of antibodies to swine vesicular disease. Research in Veterinary Science 20, 142–7.CrossRefGoogle ScholarPubMed
Lake, J. R. (1975). Studies on the genetic and biological properties of temperature-sensitive mutants of foot-and-mouth disease virus. Ph.D. thesis, University of London.Google Scholar
Mackenzie, J. S. (1969). Virulence of temperature-sensitive mutants of influenza virus. British Medical Journal iii, 757–8.CrossRefGoogle Scholar
Mowat, G. N., Prince, M. J., Spier, R. E. & Staple, R. F. (1974). Preliminary studies on the development of a swine vesicular disease vaccine. Archiv für die gesamte Virusforschung 44, 350–60.CrossRefGoogle ScholarPubMed
Pringle, C. R. (1968). Recombination between conditional lethal mutants within a strain of foot-and-mouth disease virus. Journal of General Virology 2, 199–202.CrossRefGoogle ScholarPubMed
Wright, P. F., Woodend, W. G. & Chanock, R. M. (1970). Temperature-sensitive mutants of respiratory syncytial virus: in vivo studies in hamsters. Journal of Infectious Diseases 122, 501–12.CrossRefGoogle ScholarPubMed
Zygraich, N. & Huygelen, C. (1973). In vivo behaviour of a temperature-sensitive (ts) mutant of herpesvirus hominis type 2. Archiv für die gesamte Virusforschung 43, 103–11.CrossRefGoogle ScholarPubMed
Zygraich, N., Lobmann, M. & Huygelen, C. (1972). Inoculation of hamsters with a temperature-sensitive mutant of parainfluenza 3 virus. Journal of Hygiene 70, 229–34.CrossRefGoogle ScholarPubMed