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Immunological response in cows to Mycoplasma bovigenitalium, strain 1836

Published online by Cambridge University Press:  15 May 2009

D. H. Roberts
D. H. Roberts
Affiliation:
Central Veterinary Laboratory, Weybridge, Surrey
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Four cows were inoculated via the udder with the 1836 strain of Mycoplasma bovigenitalium. The serological response was investigated using the complement-fixation and serum plate agglutination tests. A heterologous response to M. mycoides was observed, associated with an increase in the anti-γ-globulin titres in the cow's serum. The heterologous response persisted in one cow for 6 weeks after infection. The nature of the immunoglobulin response was characterized by density gradient centrifugation, and by mercaptoethanol and rivanol treatment of the serum and was found to consist entirely of 19 S γ-globulins.

The Mycoplasma persisted in the udder for the 18 weeks duration of the experiment. The Mycoplasma was not isolated from the blood. One of the cows became lame with swollen joints; Bacillus cereus was isolated from the joints and blood.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 4 , December 1968 , pp. 585 - 593
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Afshar, A., Stuart, P. & Huck, R. A. (1966). Granular vulvovaginitis (nodular venereal disease) of cattle associated with Mycoplasma bovigenitalium. Vet. Rec. 78, 512–19.CrossRefGoogle ScholarPubMed
Campbell, A. D. & Turner, A. W. (1963). Studies on contagious pleuropneumonia of cattle. IV. An improved complement-fixation test. Aust. vet. J. 29, 154–63.CrossRefGoogle Scholar
Clyde, W. A. (1964). Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immun. 92, 958–65.CrossRefGoogle ScholarPubMed
Coghlan, J. D. & Weir, D. M. (1967). Antibodies in human brucellosis. Br. med. J. ii, 269–71.CrossRefGoogle Scholar
Cowan, S. T. & Steel, K. J. (1965). Manual for the Identification of Medical Bacteria. Cambridge University Press.Google Scholar
Deutsch, H. F. & Morton, J. I. (1958). Human serum macroglobulins and dissociation units. 1. Physiochemical properties. J. biol. Chem. 231, 1107–18.CrossRefGoogle Scholar
FAO (1967). Third meeting of the FAO/OIE/OAU expert panel on contagious bovine pleuropneumonia.Khartoum, Sudan,February 1967.Google Scholar
Fernald, G. W., Clyde, W. A. & Denny, F. W. (1967). Nature of the immune response to Mycoplasma pneumoniae. J. Immun. 98, 1028–38.CrossRefGoogle ScholarPubMed
Jasper, D. E., Jain, N. C. & Brazil, B. S. (1966). Clinical and laboratory observations on bovine mastitis due to mycoplasma. J. Am. vet. med. Ass. 148, 1017–29.Google ScholarPubMed
Kunkel, H. G. (1960). Macroglobulins and high molecular weight antibodies. In ThePlasma Proteins, 1, 279–307. Ed. Putnam, F. W.. New York: Academic Press.CrossRefGoogle Scholar
Leach, R. H. (1967). Comparative studies of mycoplasma of bovine origin. Ann. N.Y. Acad. Sci. 143, 305–16.CrossRefGoogle ScholarPubMed
Patras, B. & Stone, W. H. (1961). Purification of bovine transferrins by rivanol fractionation. Fedn Proc. Fedn Am. Socs exp. Biol. 20, 74.Google Scholar
Pike, R. M. (1967). Antibody heterogeneity and serological reactions. Bact. Rev. 31, 157–74.CrossRefGoogle ScholarPubMed
Roberts, D. H. (1964). L-phase bacterial forms associated with infectious synovitis in chickens and turkeys. Res. vet. Sci. 5, 441–9.CrossRefGoogle Scholar
Roberts, D. H. & Olesiuk, O. M. (1967). Serological studies with Mycoplasma synoviae. Avian Dis. 11, 104–19.CrossRefGoogle ScholarPubMed
Roy, S. & Ghadially, F. N. (1967). Aetiological significance of rod-shaped bodies in rheumatoid synovia. Nature, Lond. 213, 1139–40.CrossRefGoogle ScholarPubMed
Schmidt, N. J., Lennette, E. H., Dennis, J. & Gee, P. S. (1966). On the nature of complement-fixing antibodies to Mycoplasma pneumoniae. J. Immun. 97, 95–9.CrossRefGoogle ScholarPubMed
Stuart, P., Davidson, I., Slavin, G., Edgson, F. A. & Howell, D. (1963). Bovine mastitis caused by mycoplasma. Vet. Rec. 75, 55–64.Google Scholar