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The immunogenic activity of ribosomal fractions derived from Brucella abortus

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
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The immunizing activity of ribosome preparations derived from Brucella abortus strain 19 cells was examined in guinea-pigs and mice. After subcutaneous injections of Br. abortus ribosomes in Freund's incomplete adjuvant, both mice and guinea-pigs developed immunity to challenge by virulent Br. abortus 544 organisms which was at least as effective as the protection conferred by live strain 19 vaccine. Both mice and guinea-pigs also developed agglutinating and complement-fixing antibodies and delayed hypersensitivity to Br. abortus antigens. Conversely, ribosome preparations elicited delayed hypersensitivity reactions on intracutaneous injection into guinea-pigs chronically infected with Br. abortus or Br. melitensis.

On injection into rabbits, Br. abortus ribosomes incorporated in incomplete adjuvant induced high titres of agglutinins, complement fixing antibodies and precipitins for Br. abortus antigens. On immunochemical examination, the ribosome preparations were not grossly contaminated with antigens derived from the cell surface. They were chemically complex, however, and in addition to RNA contained numerous protein components identified by disk electrophoresis. The nature of the components responsible for conferring protection against Br. abortus was not determined.

Type
Research Article
Information
Journal of Hygiene , Volume 76 , Issue 1 , February 1976 , pp. 65 - 74
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Boyce, K. J. & Edgar, A. W. (1966). Production of freeze-dried Brucella abortus strain 19 vaccine using cells produced by continuous culture. Journal of Applied Bacteriology 29, 401.CrossRefGoogle Scholar
Corbel, M. J. & Morris, J. A. (1974). Studies on a smooth phage-resistant variant of Brucella abortus. I. Immunological properties. British Journal of Experimental Pathology 55, 78.Google ScholarPubMed
Corbel, M. J. & Morris, J. A. (1975). Studies on a smooth phage-resistant variant of Brucella abortus. II. Mechanism of phage resistance. British Journal of Experimental Pathology 56, 1.Google ScholarPubMed
Ellwood, D. C., Keppie, J. & Smith, H. (1967). The chemical basis of the virulence of Brucella abortus. VIII. The identity of purified immunogenic material from culture filtrate and from the cell wall of Brucella abortus grown in vitro. British Journal of Experimental Pathology 48, 28.Google Scholar
Johnson, W. (1972). Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infection and Immunity 5, 947.CrossRefGoogle ScholarPubMed
Johnson, W. (1973). Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium. Infection and Immunity 8, 395.CrossRefGoogle ScholarPubMed
Mackaness, G. B. (1964). The immunological basis of acquired cellular resistance. Journal of Experimental Medicine 120, 105.CrossRefGoogle ScholarPubMed
Markenson, J., Sulitzeanu, D. & Olitzki, A. L. (1959). Immunizing properties of insoluble cell materials derived from Brucella. Nature, London 184, 1693.CrossRefGoogle Scholar
Morgan, W. J. B. & Gower, S. G. M. (1966). In Identification Methods for Microbiologists, (ed. Gibbs, B. M. and Skinner, F. A.), p. 35. New York: Academic Press.Google Scholar
Morris, J. A. (1973). The use of polyacrylamide gel electrophoresis in taxonomy of Brucella. Journal of General Microbiology 76, 231.CrossRefGoogle ScholarPubMed
Ottosen, H. E. & Plum, N. (1949). A nonantigenic allergic agent for intradermal brucellosis tests. American Journal of Veterinary Research 10, 5.Google Scholar
Paterson, J. S., Pirie, N. W. & Stableforth, A. W. (1947). Protective antigens isolated from Br. abortus. British Journal of Experimental Pathology 28, 223.Google ScholarPubMed
Ralston, D. J. & Elberg, S. S. (1971). Serum mediated immune cellular responses to Brucella melitensis. VII. The separation and assay of serum globulins responsible for macrophage stimulation and Brucella inhibition. Journal of Infectious Diseases 123, 507.CrossRefGoogle Scholar
Rasooly, G., Olitzki, A. L. & Sulitzeanu, D. (1966). Immunization against Brucella with killed vaccines. Immunizing activity in mice of Brucella cell walls and of fractions derived from them. Israel Journal of Medical Science 2, 569.Google Scholar
Smith, H., Keppie, J., Pearce, J. H. & Witt, K. (1962). The chemical basis of the virulence of Brucella abortus. IV. Immunogenic products from Brucella abortus grown in vivo and in vitro. British Journal of Experimental Pathology 43, 538.Google ScholarPubMed
Snyder, F. & Stephens, N. (1959). A simplified spectrophotometric determination of ester groups in lipids. Biochimica et biophysica acta 34, 244.Google ScholarPubMed
Sterne, M., Trim, G. & Broughton, E. S. (1971). Immunisation of laboratory animals and cattle with non-agglutinogenic extracts of Brucella abortus strain 45/20. Journal of Medical Microbiology 4, 185.CrossRefGoogle ScholarPubMed
Thomas, D. & Weiss, E. (1972). Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis. Infection and Immunity 6, 355.CrossRefGoogle ScholarPubMed
Thompson, H. C. W. & Snyder, I. S. (1971). Protection against pneumococcal infection by a ribosomal preparation. Infection and Immunity 3, 16.CrossRefGoogle ScholarPubMed
Venneman, M. R. & Berry, L. J. (1971). Experimental salmonellosis: differential passive transfer of immunity with serum and cells obtained from ribosomal and ribonucleic acid-immunized mice. Journal of the Reticuloendothelial Society 9, 491.Google ScholarPubMed
Venneman, M. R. & Bigley, N. J. (1969). Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium. Journal of Bacteriology 100, 140.CrossRefGoogle ScholarPubMed
Venneman, M. R., Bigley, N. J. & Berry, L. J. (1970). Immunogenicity of ribonucleic acid preparations obtained from Salmonella typhimurium. Infection and Immunity 1, 574.CrossRefGoogle ScholarPubMed
Winston, S. H. & Berry, L. J. (1970). Immunity induced by ribosomal extracts from Staphylococcus aureus. Journal of the Reticuloendothelial Society 8, 13.Google ScholarPubMed
Youmans, A. S. & Youmans, G. P. (1965). Immunogenic activity of a ribosomal fraction obtained from Mycobacterium tuberculosis. Journal of Bacteriology 89, 1291.CrossRefGoogle ScholarPubMed
Youmans, A. S. & Youmans, G. P. (1966 a). Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis. Journal of Bacteriology 91, 2146.CrossRefGoogle ScholarPubMed
Youmans, A. S. & Youmans, G. P. (1966 b). Preparation of highly immunogenic ribosomal fractions of Mycobacterium tuberculosis by the use of sodium dodecyl sulfate. Journal of Bacteriology 91, 2139.CrossRefGoogle ScholarPubMed