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Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
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Summary

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The antibodies active in the Rose Bengal plate test (RBPT) for bovine brucellosis have been studied. The results of fractionation experiments showed that RBPT activity was associated with fractions containing immunoglobulin of the IgG1 class; other immunoglobulin classes were inactive in this respect although active in other tests. These results were confirmed by inhibition tests with specific antisera and by elution of the antibody from agglutinated RBPT antigen.

The major proportion of the serum complement-fixing activity was also present in the IgG1 fraction and it is suggested that the RBPT and CF reactions are probably mediated by the same antibodies.

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 4 , December 1972 , pp. 779 - 795
Copyright
Copyright © Cambridge University Press 1972

References

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