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Hybrids derived from the viruses of variola major and cowpox

Published online by Cambridge University Press:  15 May 2009

H. S. Bedson  and
K. R. Dumbell
H. S. Bedson
Affiliation:
Department of Bacteriology, University of Liverpool
K. R. Dumbell
Affiliation:
Department of Bacteriology, University of Liverpool
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The characterization of 16 clones of virus derived from mixed infections with variola major and cowpox has been described. This work has involved the description of the plaques produced by variola major in chick embryo monolayers, and a more detailed study of the effects of raised temperature on pock production by pox viruses on the C.A.M.

Two of the variola major—cowpox clones were found to correspond to cowpox virus, while the other 14 shared various combinations of parental characters. Among them there were 10 distinct new types of virus. Evidence of the stability of these viruses has been presented.

Analysis of the combination of characters encountered among the hybrids has given good evidence that all the eight marker characters used segregate independently. A particular instance of this was the character of chick embryo virulence which, among the hybrid viruses, was shown to be unrelated either to ceiling temperature or speed of plaque formation.

The significance of these findings in relation to the general problem of virulence has been briefly discussed. The possibility that vaccinia may have originated as a hybrid of variola and cowpox has lso been considered.

Type
Research Article
Information
Journal of Hygiene , Volume 62 , Issue 2 , June 1964 , pp. 147 - 158
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Bedson, H. S. & Dumbell, K. R. (1961). The effect of temperature on the growth of pox viruses in the chick embryo. J. Hyg., Camb., 59, 457.Google ScholarPubMed
Bedson, H. S. & Dumbell, K. R. (1964). Hybrids derived from the viruses of alastrim and rabbit pox. J. Hyg., Camb., 62, 141.CrossRefGoogle ScholarPubMed
Downie, A. W. (1939). A study of the lesions produced experimentally by cowpox virus. J. Path. Bact. 48, 361.CrossRefGoogle Scholar
Downie, A. W. & Dumbell, K. R. (1956). Pox viruses. Annu. Rev. Microbiol. 10, 237.CrossRefGoogle ScholarPubMed
Downie, A. W. & Haddock, D. W. (1952). A variant of cowpox virus. Lancet, i, 1049.CrossRefGoogle Scholar
Dumbell, K. R. & Bedson, H. S. (1964). The use of ceiling temperature and reactivation in the isolation of pox virus hybrids. J. Hyg., Camb., 62, 133.CrossRefGoogle ScholarPubMed
Fenner, F. & Comben, B. M. (1958). Genetic studies with mammalian poxviruses. I. Demonstration of recombination between two strains of vaccinia virus. Virology, 5, 530.CrossRefGoogle Scholar
Gemmell, A. & Cairns, J. (1959). Linkage in the genome of an animal virus. Virology, 8, 381.CrossRefGoogle ScholarPubMed
Gemmell, A. & Fenner, F. (1960). Genetic studies with mammalian poxviruses. III. White (u) mutants of rabbitpox virus. Virology, 11, 219.CrossRefGoogle Scholar
Mayr, A. & Kalcher, K. (1961). Plaque-bildung bei den geflügelpockenviren. Arch. ges. Virusforsch. 11, 307.CrossRefGoogle Scholar
Mika, L. A. & Pirsch, J. B. (1960). Differentiation of variola from other members of the poxvirus group by the plaque technique. J. Bact. 80, 861.CrossRefGoogle ScholarPubMed
Pettenkofer, H. J., Stöss, B., Helmbold, W. & Vogel, F. (1962). Alleged causes of the present-day world distribution of the human ABO blood groups. Nature, Lond., 193, 445.CrossRefGoogle ScholarPubMed
Rondle, C. J. M. & Dumbell, K. R. (1962). Antigens of cowpox virus. J. Hyg., Camb., 60, 41.CrossRefGoogle ScholarPubMed
Shedlovsky, T. & Smadel, J. E. (1942). The LS-antigen of vaccinia. II. Isolation of a single substance containing both L- and S-activity. J. exp. Med. 75, 165.CrossRefGoogle Scholar