Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-22T15:49:23.383Z Has data issue: false hasContentIssue false
  • English
  • Français

Host–microflora interaction in Systemic Lupus Erythematosus (SLE): colonization resistance of the indigenous bacteria of the intestinal tract

Published online by Cambridge University Press:  15 May 2009

H. Z. Apperloo-Renkema ,
H. Bootsma ,
B. I. Mulder ,
C. G. Kallenberg  and
D. Van Der Waaij
H. Z. Apperloo-Renkema
Affiliation:
Laboratory for Medical Microbiology
H. Bootsma
Affiliation:
Department of Clinical Immunology, State University Oostersingel 59, Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
B. I. Mulder
Affiliation:
Department of Clinical Immunology, State University Oostersingel 59, Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
C. G. Kallenberg
Affiliation:
Department of Clinical Immunology, State University Oostersingel 59, Groningen, Oostersingel 59, 9713 EZ, Groningen, The Netherlands
D. Van Der Waaij
Affiliation:
Laboratory for Medical Microbiology
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Experimental data suggest a role for the microflora in Systemic Lupus Erythematosus (SLE). Anti–ds–DNA antibodies may be pathogenic in SLE by forming immune complexes with DNA. Foreign bacteria in the intestines could constitute the stimulus for anti–ds–DNA antibody production in SLE. Colonization Resistance (CR) is the defence capacity of the indigenous microflora against colonization of the intestines by foreign bacteria. A low CR implies increase of translocation of bacteria and a higher chance of subsequent, possibly DNA–cross–reacting antibacterial antibody production.

We measured CR by a comprehensive biotyping technique in healthy individuals and patients with inactive and active SLE. CR tended to be lower in active SLE patients than in healthy individuals (P = 0.09, Wilcoxon one sided, with correction for ties). This could indicate that in SLE more and different bacteria translocate across the gut wall due to a lower CR. Some of these may serve as polyconal B cell activators or as antigens cross-reacting with DNA.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 2 , April 1994 , pp. 367 - 373
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

Tan, EM. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol 1982: 33: 167–240.Google ScholarPubMed
Borg, EJ ter, Horst, G, Hummel, EJ, Limburg, PC, Kallenberg, CGM. Measurement of increases in anti–double–stranded DNA antibody levels as a predictor of disease exacerbation in systemic lupus erythematosus. Arthritis Rheum 1990: 33: 634–43.CrossRefGoogle ScholarPubMed
Waaij, D van der. The composition of the microflora, functional aspects of the intestinal immune system and auto–immune phenomena. In: Sazaki, S. ed. Recent advances in germfree research. Tokai: Tokai University Press, 1981: 387–95.Google Scholar
Penhale, WJ, Young, PR. The influence of the normal microbial flora on the susceptibility of rats to experimental autoimmune thyroiditis. Clin Exp Immunol 1988; 72: 288–92.Google ScholarPubMed
Lieberum, B, Hartman, K. Successive changes of the cellular composition in lymphoid organs of MLR–MP/lpr–lpr mice during the development of lymphoproliferative disease as investigated in cryosections. Clin Immunol Immunopathol 1988; 46: 421–31.CrossRefGoogle Scholar
Murosaki, S, Yoshikai, Y, Kubo, C et al. . Influence of intake of skim milk from cows immunized with intestinal bacterial antigens on onset of renal disease in (NZB/NZW)F1 mice fed ad libitum or restricted in energy intake. J Nutr 1991: 121: 1860–8.CrossRefGoogle ScholarPubMed
Terada, K, Okuhara, E, Kawarada, Y. Antigen DNA isolated from immune complexes in plasma of patients with systemic lupus erythematosus hybridizes with the Escherichia coli lac Z gene. Clin Exp Immunol 1991; 85: 66–9.CrossRefGoogle ScholarPubMed
Klinman, DM. Regulation of B cell activation in autoimmune mice. Clin Immunol Immunopathol 1989; 53: S25–S34.Google Scholar
Klinman, DM. Polyclonal B cell activation in lupus–prone mice precedes and predicts the development of autoimmune disease. J Clin Invest 1990: 86: 1249–54.Google Scholar
Borg, EJ ter, Horst, G, Hummel, E, Limburg, PC, Kallenberg, CGM. Rises in anti–double stranded DNA antibody levels prior to exacerbations of systemic lupus erythematosus are not merely due to polyclonal B cell activation. Clin Immunol Immunopathol 1991: 59: 117–28.Google Scholar
Carroll, P, Stafford, D, Schwartz, RS, Stollar, BD. Murine monoclonal anti–DNA autoantibodies bind to endogenous bacteria. J Immunol 1985: 135: 1086–90.CrossRefGoogle ScholarPubMed
Shoenfeld, Y, Teplizki, HA, Mendlovic, S, Blank, M, Mozes, E, Isenberg, DA. The role of the human anti–DNA idiotype 16/6 in autoimmunity. Clin Immunol Immunopathol 1989: 51: 313–25.CrossRefGoogle ScholarPubMed
Grayzel, A, Solomon, A, Aranow, C, Diamond, B. Antibodies elicited by pneumococcal antigens bear an anti–DNA–associated idiotype. J Clin Invest 1991: 87: 842–6.Google Scholar
Harkiss, GD, Hendric, F, Nuki, G. Ligand-binding and idiotype cross–reactions between anti–DNA antibodies and antibodies to Klebsiella K30 polysaccharide in patients with systemic lupus erythematosus or rheumatoid arthritis. Clin Immunol Immunopathol 1987: 44: 283–96.CrossRefGoogle ScholarPubMed
Dziarski, R. Autoimmunity: polyclonal activation or antigen induction? Immunol Today 1988; 9: 340–2.CrossRefGoogle ScholarPubMed
Pisetsky, DS, Grudier, JP, Gilkeson, GS. A role for immunogenic DNA in the pathogenesis of systemic lupus erythematosus. Arthritis Rheum 1990: 33: 153–9.CrossRefGoogle ScholarPubMed
Waaij, D van der, Berghuis–de Vries, JM, Lekkerkerk–van der Wees, JEC. Colonization resistance of the digestive tract in conventional and antibiotic–treated mice. J Hyg 1971; 69: 405–11.CrossRefGoogle ScholarPubMed
Waaij, D van der, Berghuis-de Vries, JM, Lekkerkerk–van der Wees, JEC. Colonization resistance of the digestive tract and the spread of bacteria to the lymphatic organs in mice. J Hyg 1972: 70: 335–42.Google Scholar
Berg, RD. Inhibition of Escherichia coli translocation from the gastrointestinal tract by normal cecal flora in gnotobiotic or antibiotic decontaminated mice. Infect Immun 1980: 3: 1073–81.Google Scholar
Trancrede, CH, Andremont, AO. Bacterial translocation and gram-negative bacteremia in patients with hematological malignancies. J Infect Dis 1985: 152: 99–103.CrossRefGoogle Scholar
Lescut, D, Colombel, JF, Vincent, P et al. , Bacterial translocation in colorectal cancers. Gastroenterol Clin Biol 1990: 14: 811–14.Google ScholarPubMed
Brathwaite, CE, Ross, SE, Nagele, R, Mure, AJ, O'Malley, KF, Garcia-Perez, FA. Bacterial translocation occurs in humans after traumatic injury: evidence using immunofluorescence. J Trauma 1993: 34: 586–9.CrossRefGoogle ScholarPubMed
Manson, WL, Westerveld, AW, Klasen, HJ, Sauer, EW. Selective intestinal decontamination of the digestive tract for infection prophylaxis in severely burned patients. Scand J Plast Reconstr Surg 1987: 21: 269–72.Google ScholarPubMed
Apperloo-Renkema, HZ, Waaij, D van der. Study of colonization resistance for Enterobacteriaceae in man by experimental contamination and biotyping as well as the possible role of antibodies in the clearance of these bacteria from the intestines. Epidemiol Infect 1991: 107: 619–26.Google Scholar
Apperloo-Renkema, HZ, Wilkinson, MHF, Waaij, D van der. Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis. Epidemiol Infect 1992: 109: 497–506.Google Scholar
Liang, MH, Socher, SA, Larson, MG, Schur, PH. Reliability and validity of six systems for the clinical assessment of disease activity in systemic lupus erythematosus. Arthritis Rheum 1989; 32: 1107–18.CrossRefGoogle ScholarPubMed
Waaij, D van der, Berghuis, JM. Determination of the colonization resistance of the digestive tract of individual mice. J. Hyg 1974: 72: 379–87.Google Scholar
Apperloo-Renkema, HZ, Waaij, BD van der, Waaij, D van der. Determination of colonization resistance of the digestive tract by biotyping of Enterobacteriaceae. Epidemiol Infect 1990: 105: 355–61.CrossRefGoogle ScholarPubMed
Waaij, D van der, Tielemans-Speltie, TM, Roeck de-Houben, AMJ. Infection by and distribution of biotypes of Enterobacteriaceae species in leukaemic patients treated under ward conditions and in units for protective isolation in seven hospitals in Europe. Infection 1977: 5: 188–94.CrossRefGoogle ScholarPubMed