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High resolution genotyping of Campylobacter jejuni strains by macrorestriction analysis with XhoI and polymerase chain reaction targeting enterobacterial repetitive intergenic consensus sequences: can we predict the zoonotic potential of strains?

Published online by Cambridge University Press:  14 February 2003

I. MOSER ,
P. LENTZSCH ,
B. RIEKSNEUWOEHNER ,
P. SCHWERK  and
L. H. WIELER
I. MOSER
Affiliation:
Institut für Mikrobiologie und Tierseuchen, Freie Universität, Berlin, Germany
P. LENTZSCH
Affiliation:
Zentrum für Agrarlandschafts- und Landnutzungsforschung (ZALF) e.V., Müncheberg, Germany
B. RIEKSNEUWOEHNER
Affiliation:
Institut für Mikrobiologie und Tierseuchen, Freie Universität, Berlin, Germany
P. SCHWERK
Affiliation:
Institut für Mikrobiologie und Tierseuchen, Freie Universität, Berlin, Germany
L. H. WIELER
Affiliation:
Institut für Mikrobiologie und Tierseuchen, Freie Universität, Berlin, Germany
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Abstract

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Campylobacter jejuni isolates of human, canine, feline, bovine and poultry origin were investigated for their genomic diversity using O-antigen typing (n = 271), SmaI (n = 158) and XhoI (n = 158) macrorestriction analysis and ERIC–PCR (n = 107). The O-antigens O[ratio ]1/44, O[ratio ]2, O[ratio ]4 complex, O[ratio ]37, O[ratio ]40 were identified and 53.7% of the human and 56.1% of the animal strains were typable with the available antisera. Two ERIC–PCR pattern groups were generated representing human and animal strains as well as those exclusively of animal origin. XhoI macrorestriction analysis also distinguished ‘human’ and ‘non-human’ strain clusters, but by SmaI restriction mainly serotype-associated clusters were found. In conclusion, genomic differences may occur between ‘human’ and ‘non-human’ strains and this may reflect their potential to overcome the barrier from animals to humans.

Type
Research Article
Information
Epidemiology & Infection , Volume 129 , Issue 3 , December 2002 , pp. 435 - 443
Copyright
2002 Cambridge University Press