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High prevalence of thermostable direct hemolysin (TDH)-like toxin in Vibrio mimicus strains isolated from diarrhoeal patients

Published online by Cambridge University Press:  15 May 2009

M. Uchimura ,
K. Koiwai ,
Y. Tsuruoka  and
H. Tanaka
M. Uchimura
Affiliation:
Public Health Laboratory of Chiba Prefecture, 666–2 Nitona-cho, Chuo-ku, Chiba, 260 Japan
K. Koiwai
Affiliation:
Public Health Laboratory of Chiba Prefecture, 666–2 Nitona-cho, Chuo-ku, Chiba, 260 Japan
Y. Tsuruoka
Affiliation:
Public Health Laboratory of Chiba Prefecture, 666–2 Nitona-cho, Chuo-ku, Chiba, 260 Japan
H. Tanaka
Affiliation:
Institute of Medical Science, the University of Tokyo 4-6 Minatoku, Tokyo, 108
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Summary

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A total of 17 isolates of Vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. Sixteen (94%) clinical isolates and one (50%) from food produced TDH-like toxin, whereas none of the environmental isolates did so. The food from which V. mimicus with TDH-like toxin production was isolated, was one which had caused food poisoning. Only one environmental strain produced CT-like toxin, whilst ST-like toxin was not detected from any strains tested.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 1 , August 1993 , pp. 49 - 53
Copyright
Copyright © Cambridge University Press 1993

References

1.Davis, BR, Fanning, GR, Madden, JM, Steigerwalt, AG Jr., Smith, HL Jr., Brenner, DJ. Characterization of biochemically atypical Vibrio cholerae strains and designation of a new pathogenic species. Vibrio mimicus. J Clin Microbiol 1981; 14: 631–9.CrossRefGoogle ScholarPubMed
2.Shandera, WX, Johnston, JM, Davis, BR, Blake, PA. Disease from infection with Vibrio mimicus, a newly recognized Vibrio species. Ann Intern Med 1983; 99: 169–71.CrossRefGoogle ScholarPubMed
3.Spira, WM, Fedorka-Cray, PJ. Purification of enterotoxins from Vibrio mimicus that appear to be identical to cholera toxin. Infect Immun 1984; 45: 169–84.CrossRefGoogle ScholarPubMed
4.Arita, M, Honda, T, Miwatani, T, Takeda, T, Takao, T, Shimonishi, Y. Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus. FEMS Microbiol Lett 1991; 79: 105–10.CrossRefGoogle Scholar
5.Chowdhury, MAR, Aziz, KMS, Kay, BA, Rahim, Z. Toxin production by Vibrio mimicus strain isolated from human and environmental sources in Bangladesh. J Clin Microbiol 1987; 25: 2200–3.CrossRefGoogle ScholarPubMed
6.Honda, T, Miwatani, T. Multi-toxigenicity of Vibrio cholerae Non-O1. In Advances in research on cholera and related diarrheas. Ohtomo, N, Sack, RB, eds., vol. 6. Tokyo: KTK Scientific Publishers, 1988: 23–32.Google Scholar
7.Nishibuchi, M, Khaeomanee-iam, V, Honda, T, Kaper, JB, Miwatani, T. Comparative analysis of the hemolysin genes of Vibrio cholerae non-O1, V. mimicus, and V. hollisae that are similar to the tdh gene of V. parahaemolyticus. FEMS Microbiol Lett 1990; 67: 251–6.CrossRefGoogle Scholar
8.Tada, J, Ohashi, T, Nishimura, N, et al. Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction. Mol Cell Prob 1992; 6: 477–87.CrossRefGoogle ScholarPubMed
9.Nischibuchi, M, Seidler, RJ. Medium-development production of extracellular enterotoxins by Non-O1 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvilais. Appl Environ Microbiol 1983; 45: 228–31.CrossRefGoogle Scholar
10.Dean, AG, Ching, YC, Williams, RG, Harden, LB. Test of Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis 1982; 125: 407–11.CrossRefGoogle Scholar
11.Uesaka, Y, Otsuka, Y, Kashida, M, et al. Detection of cholera toxin by highly sensitive bead-Enzyme Liked Immunosorbent Assay. Microbiol Immunol 1992; 36: 43–53.CrossRefGoogle Scholar
12.Oku, Y, Uesaka, Y, Hirayama, T, Takeda, Y. Development of a highly sensitive bead-ELISA to detect bacterial protein toxins. Microbiol Immunol 1988; 32: 807–16.CrossRefGoogle ScholarPubMed
13.Miyamoto, Y, Kato, T, Obara, Y, Akyama, S, Takizawa, K, Yamai, S. In vitro hemolytic characteristic of Vibrio paraheamolyticus: Its close correlation with human pathogenicity. J Bacteriol 1969; 100: 1147–9.CrossRefGoogle Scholar
14.Sakazaki, R, Tamura, K, Kato, T, Obara, Y, Yamai, S, Hobo, K. Studies on the enteropathogenic, facultatively halophilic bacteria. Vibrio parahaemolyticus. III. Enteropathogenicity. Japan J Med Sci Biol 1968; 21: 325–31.CrossRefGoogle Scholar
15.Nishibuchi, M, Fasano, A, Russell, RG, Kaper, JB. Enterotoxigenicity of Vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin. Infect Immun 1992; 60: 3539–45.CrossRefGoogle ScholarPubMed