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Haemophilus influenzae subtyping by SDS-PAGE of whole-cell polypeptides

Published online by Cambridge University Press:  19 October 2009

A. J. Paterson ,
K. F. Macsween  and
T. H. Pennington
A. J. Paterson
Affiliation:
Department of Bacteriology, University of Aberdeen, Foresterhill, Aberdeen
K. F. Macsween
Affiliation:
Department of Bacteriology, University of Aberdeen, Foresterhill, Aberdeen
T. H. Pennington
Affiliation:
Department of Bacteriology, University of Aberdeen, Foresterhill, Aberdeen
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Summary

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Strains of Haemophilus influenzae isolated from patients in N.E. Scotland between 1983 and 1986 have been subtyped by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell polypeptides. Gels were stained with Coomassie blue and polypeptide profiles were analysed using the Dice coefficient of similarity.

Type b strains were all closely related, the 19 strains analysed being grouped at a 90% similarity level into one large (13 strains) and one small (3 strains) cluster with 3 strains being ungrouped. Thirty-six non-typable, epidemiologically unrelated strains were subtyped; one pair of strains had indistinguishable polypeptide profiles. The polypeptide profiles of the remaining strains showed much heterogeneity, although groups of strains isolated from the same patient over short periods showed indistinguishable profiles.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 179 - 189
Copyright
Copyright © Cambridge University Press 1987

References

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