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Published online by Cambridge University Press: 15 May 2009
The culture of organisms in a stagnant liquid medium, which is the normal method employed when the products of their metabolism are studied, has obvious disadvantages. The medium is constantly varying in its constituents and physical characteristics; prominent variations are oxygen tension, pH, food supplies and surface tension, and growth phase of the organism. Experimenters are constantly occupied in preparing nutritive media bearing closer analogy to living tissue fluids; but strangely enough little attention has been directed to maintaining the dynamic conditions obtaining when infection takes place. Yet such conditions may greatly modify the metabolism and general pathological activity of the organism.