Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-28T21:47:28.813Z Has data issue: false hasContentIssue false

Growth of rubella virus in human embryonic organ cultures

Published online by Cambridge University Press:  15 May 2009

Jennifer M. Best
Affiliation:
Clinical Virology Laboratory, St Thomas's Hospital, London, S.E. 1
J. E. Banatvala
Affiliation:
Clinical Virology Laboratory, St Thomas's Hospital, London, S.E. 1
Barbara M. Moore
Affiliation:
Clinical Virology Laboratory, St Thomas's Hospital, London, S.E. 1
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two strains of rubella virus multiplied in organ cultures of human embryonic trachea, nasal epithelium, pharynx, larynx, skin and brain derived from foetuses aged 15–28 weeks. Growth curve experiments conducted on cultures of nasal epithelium and trachea showed that virus appeared in the culture fluid 72 hr. after inoculation and thereafter rose to titres varying from 10 to 103·7TCD50/ml. These titres persisted for periods up to 34 days after inoculation. Intracelmlar and organ culture fluid virus titres were shown to be similar in specimens tested in both the early and late stages of the growth curve. No degenerative changes or loss of ciliary activity was observed in these cultures.

We are grateful to Dr H. E. M. Kay and his staff (Royal Marsden Hospital), for the supply of human embryos; Dr D. A. J. Tyrrell (M.R.C. Common Cold Research Unit, Salisbury) for his encouragement, and Miss G. E. Fairbairn (St Thomas’s Hospital) for the histological preparations.

This research was supported by a grant from the Medical Research Council.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1968

References

Albanese, M. & Romano, N. (1967). Effects of rubella virus on tracheal organ cultures. Riv. Ist. sieroter, ital. 42, 217.Google ScholarPubMed
American Pediatric Society & Society for Pediatric Research (1965). Rubella symposium. Am. J. Dis. Child. 110, 345.Google Scholar
Banatvala, J. E., Horstmann, D. M., Payne, M. C. & Gluck, L. (1965). Rubella syndrome and thrombocytopenic purpura in newborn infants. New Engl. J. Med. 273, 474.CrossRefGoogle ScholarPubMed
Best, J. M. & Banatvala, J. E. (1967). A comparison of RK 13, vervet monkey kidney and patas monkey kidney cell cultures for the isolation of rubella virus. J. Hyg., Gamb. 65, 263.CrossRefGoogle ScholarPubMed
Best, J. M., Banatvala, J. E., Almeida, J. & Waterson, A. P. (1967). Morphological characteristics of rubella virus. Lancet, ii, 237.CrossRefGoogle Scholar
Gregg, N. M. (1941). Congenital cataract following German measles in mother. Trans. ophthal. Soc. Aust. 3, 35.Google Scholar
Holmes, I. H. & Warburton, M. F. (1967). Is Rubella an Arbovirus? Lancet, ii, 1233.CrossRefGoogle Scholar
Hoorn, B. & Tyrrell, D. A. J. (1965). On the growth of certain ‘newer’ respiratory viruses in organ culture. Br. J. exp. Path. 46, 109.Google Scholar
Kistler, G. S., Best, J. M., Banatvala, J. E. & Töndury, G. (1967). Elektronen-mikro-skopische Untersuchungen an rötelninfizierten menschlichen Organkulturen. Schweiz. med. Wschr. 42, 1377.Google Scholar
Lundström, R. (1952). Rubella during pregnancy: its effects upon perinatal mortality, the incidence of congenital abnormalities and immaturity. A preliminary report. Acta Paediat., Stockh. 41, 583.CrossRefGoogle ScholarPubMed
Lundström, R. (1962). Rubella during pregnancy. A follow-up study of children born after an epidemic of rubella in Sweden 1951. With additional investigations on prophylaxis and treatment of maternal rubella. Acta Paediat., Stockh., Suppl. 133.CrossRefGoogle Scholar
Manson, M. M., Logan, W. P. D. & Loy, R. M. (1960). Rubella and other virus infections during pregnancy. Rep. publ. Hlth med. Subj., Lond. 101.Google Scholar
Mccarthy, K. & Taylor-Robinson, C. H. (1965). Growth and cytopathic effects of rubella virus in primary rabbit tissue culture. Arch. ges. Virusforsch. 16, 415.CrossRefGoogle ScholarPubMed
Mcintosh, K., Dees, J. H., Becker, W. B., Kapikian, A. Z. & Chanock, R. M. (1967). Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. natn. Acad. Sci. U.S.A. 57, 933.Google Scholar
Oxford, J. S. & Schild, G. C. (1967). The evaluation of antiviral compounds for rubella virus using organ cultures. Arch. ges. Virusforsch. 22, 349.Google Scholar
Tyrrell, D. A. J. & Bynoe, M. L. (1965). Cultivation of a common cold virus in organ culture. Br. med. J. i, 1467.CrossRefGoogle Scholar
Tyrrell, D. A. J. & Bynoe, M. L. (1966). Cultivation of viruses from a high proportion of patients with colds. Lancet, i, 76.Google Scholar
Tyrrell, D. A. J. & Hoorn, B. (1966). Effects of some viruses on ciliated cells. Am. Rev. reap. Dis. 93, 156.Google Scholar