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The growth in vitro of vaccinia virus in chick embryo chorio-allantoic memberane, minced embryo and cell suspensions

Published online by Cambridge University Press:  15 May 2009

H. B. Maitland  and
D. I. Magrath
H. B. Maitland
Affiliation:
Department of Bacteriology, University of Manchester
D. I. Magrath
Affiliation:
Department of Bacteriology, University of Manchester
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The growth curve of rabbit skin-adapted vaccinia virus in the chick chorioallantoic membrane incubated in Hanks' solution showed a drop in titre of virus for about 10 hr. followed by growth. At least 25% of virus, sometimes more, remained infective. A similar fall in titre was observed in heated membranes in which the virus did not grow and this occurred also when membranes, either normal or heated, were infected and disintegrated before incubation.

The growth curve of virus in minced chick-embryo was similar to that in chorioallantoic membrane.

Virus in cell suspensions prepared from chick embryo and incubated in a nutrient medium showed only a small loss of infectivity before growth in some experiments and rarely dropped below 65–70 % of the original titre in others.

These results throw considerable doubt on the view that loss of infectivity preceding growth of vaccinia virus should be interpreted as an essential part of a growth cycle.

Type
Research Article
Information
Journal of Hygiene , Volume 55 , Issue 3 , September 1957 , pp. 347 - 360
Copyright
Copyright © Cambridge University Press 1957

References

Anderson, S. G. (1954). The growth curve of vaccinia virus on the chorioallantois. Aust. J. exp. Biol. med. Sci. 32, 633.CrossRefGoogle ScholarPubMed
Bang, F. (1950). Cellular changes in the chick chorio-allantoic membrane infected with herpes simplex and vaccinia. A study with thin sections for the electron microscope. Johns Hopk. Hosp. Bull. 87, 511.Google ScholarPubMed
Bland, J. O. W. & Robinow, C. F. (1939). The inclusion bodies of vaccinia and their relationship to the elementary bodies studied in cultures of the rabbit's cornea. J. Path. Bact. 48, 381.CrossRefGoogle Scholar
Briody, B. A. & Stannard, C. (1951). Studies on vaccinia virus. I. The development of haemagglutinating and infective particles in the chorioallantois of the chick embryo. J. Immunol. 67, 403.CrossRefGoogle ScholarPubMed
Crawford, G. N. C. & Sanders, F. K. (1952). The multiplication of vaccinia virus in tissue cultures of adult rabbit skin. Quart. J. micr. Sci. 93, 119.Google Scholar
Dulbecco, R. & Vogt, M. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. med. 99, 167.CrossRefGoogle ScholarPubMed
Flewett, T. H. (1956). Intracellular growth of some viruses of the pox group. An electron microscopic study of infected chick chorionic cells. J. Hyg., Camb., 54, 393.CrossRefGoogle ScholarPubMed
Gaylord, W. H. & Melnick, J. L. (1953). Intracellular forms of pox viruses as shown by the electron microscope (vaccinia, ectromelia, molluscum contagiosum). J. exp. Med. 98, 157.CrossRefGoogle ScholarPubMed
Hoagland, C. L., Smadel, J. E. & Rivers, T. M. (1940). Constituents of elementary bodies of vaccinia. I. Certain basic analyses and observations on lipid components of the virus. J. exp. Med. 71, 737.CrossRefGoogle ScholarPubMed
Maitland, H. B. & Tobin, B. M. (1956). The growth of vaccinia virus in the chorio-allantois of the developing chick embryo and the production of complement-fixing antigen and haemagglutinin. J. Hyg., Camb., 54, 102.CrossRefGoogle Scholar
Morgan, C., Ellison, S. A., Rose, H. M. & Moore, D. H. (1954). Structure and development of viruses observed in the electron microscope. II. Vaccinia and fowl pox viruses. J. exp. Med. 100, 301.CrossRefGoogle ScholarPubMed
Noyes, W. F. & Watson, B. K. (1955). Studies on the increase of vaccine virus in cultured human cells by means of the fluorescent antibody technique. J. exp. Med. 102, 237.CrossRefGoogle ScholarPubMed
Peters, D. & Nasemann, T. (1953). Untersuchungen am Virus der Variola-Vaccine. II. Mitt. Nachweis von Elementarkörperstadien mittels enzymatisch-elektronenoptischen Analyse. Z. Naturf. 8B, 547.CrossRefGoogle Scholar
Wyckoff, R. W. G. (1951). The virus of vaccinia in chick embryo membrane. Proc. nat. Acad. Sci., Wash., 37, 565.CrossRefGoogle ScholarPubMed