Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-22T16:16:14.580Z Has data issue: false hasContentIssue false
  • English
  • Français

Growth in chick chorioallantoic membranes of strains of Newcastle disease virus of differing virulence

Published online by Cambridge University Press:  15 May 2009

P. Reeve ,
Margaret Rosenblum  and
D. J. Alexander
P. Reeve
Affiliation:
Department of Virology, Royal Postgraduate Medical School, London, W. 12
Margaret Rosenblum
Affiliation:
Department of Virology, Royal Postgraduate Medical School, London, W. 12
D. J. Alexander
Affiliation:
Department of Virology, Royal Postgraduate Medical School, London, W. 12
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The growth of eight strains of Newcastle disease virus in chick embryo chorioallantoic membranes was studied by comparing, at different times after infection, the amounts of haemagglutinin released into the allantoic fluid (extracellular haemagglutinin) with that associated with the membrane (cell-associated haemagglutinin). The virulence of the strains examined differed in that some killed chick embryos more rapidly than others. All strains released similar amounts of extracellular haemagglutinin and maximum titres were achieved about 12 hr. after infection. With virulent strains cell-associated haemagglutinin titres increased exponentially until the death of the host and maximum titres were much higher than those of extracellular haemagglutinin. With avirulent strains cell-associated haemagglutinin titres increased exponentially for only a limited time and titres were always lower than the titres of extracellular haemagglutinin.

Similar results were obtained when the titres of neuraminidase and viral ribonucleoprotein were measured during the growth of two virulent and two avirulent strains. Virulence appears to be associated with the continued intracellular accumulation of viral antigens.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 1 , March 1970 , pp. 61 - 69
Copyright
Copyright © Cambridge University Press 1970

References

Aminoff, D. (1961). Methods for quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochemical Journal 81, 384.CrossRefGoogle ScholarPubMed
Asplin, F. D. (1952). Immunisation against Newcastle disease with a virus of low virulence (strain F) and observations on sub-clinical infection in partially resistant fowls. Veterinary Record 64, 245.Google Scholar
Compans, R. W., Holmes, , Kathryn, V., Dales, S. & Choppin, P. W. (1966). An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5. Virology 30, 411.CrossRefGoogle ScholarPubMed
Dobson, N. (1939). Newcastle Disease. Proceedings of the 7th World's Poultry Congress, Cleveland, p. 250.Google Scholar
Graham, E. R. B. (1961). Some aspects of the structure of the carbohydrate moiety of fetuin. Australian Journal of Science 24, 140.Google Scholar
Granoff, A. (1959). Studies on mixed infection with Newcastle disease virus. Virology 9, 636.CrossRefGoogle ScholarPubMed
Kingsbury, D. W. & Darlington, R. W. (1968). Isolation and properties of Newcastle disease virus nucleocapsid. Journal of Virology 2, 248.CrossRefGoogle ScholarPubMed
Pennington, T. H. (1967). Studies with Newcastle disease virus. Ph.D. Thesis, University of London.Google Scholar
Plagemann, P. W. (1968). Mengovirus replication in Novikoff rat hepatoma and mouse L cells: Effects on synthesis of host-cell macromolecules and virus specific synthesis of ribonucleic acid. Journal of Virology 2, 461.CrossRefGoogle ScholarPubMed
Schäfer, W., Schramm, G. & Traub, E. (1949). Untersuchungen über das Virus der a typischen Geflügelpest. Zeitschrift für Naturforschung 46, 157.CrossRefGoogle Scholar
Schloer, G. M. & Hanson, R. P. (1968). Relationship of plaque size and virulence for chickens of 14 representative Newcastle disease virus strains. Journal of Virology 2, 40.CrossRefGoogle ScholarPubMed
Waterson, A. P. (1968). Introduction to Animal Virology. 2nd ed., Cambridge University Press.Google Scholar
Waterson, A. P. & Cruickshank, J. G. (1963). The effect of ether on Newcastle disease virus: a morphological study of eight strains. Zeitschrift für Naturforschung 18B, 114.CrossRefGoogle Scholar
Waterson, A. P., Pennington, T. H. & Allan, W. H. (1967). Virulence in Newcastle disease virus. A preliminary study. British Medical Bulletin 23 (2), 138.CrossRefGoogle ScholarPubMed
Wilson, D. E. (1968). Inhibition of host-cell protein and RNA synthesis by Newcastle disease virus. Journal of Virology 2, 1.CrossRefGoogle ScholarPubMed