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Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal

Published online by Cambridge University Press:  15 May 2009

F. Bacellar ,
R. L. Regnery ,
M. S. Núncio  and
A. R. Filipe
F. Bacellar
Affiliation:
Centra de Estudos de Vectores e Doenças Infecciosas do Institute National de Saúde, 2965 Áquas de Moura, Portugal
R. L. Regnery
Affiliation:
Centers for Disease Control and Prevention, Division of Viral and Rickettsial Diseases, US Department of Health and Human Services, Public Health Service, Atlanta, Georgia 3033, USA
M. S. Núncio
Affiliation:
Centra de Estudos de Vectores e Doenças Infecciosas do Institute National de Saúde, 2965 Áquas de Moura, Portugal
A. R. Filipe*
Affiliation:
Centra de Estudos de Vectores e Doenças Infecciosas do Institute National de Saúde, 2965 Áquas de Moura, Portugal Escola National de Saúde Pública, Universidade Nova de Lisboa, 1699 Lisboa, Portugal
*
*Professor Armindo R. Filipe, Centre de Estudos de Vectores e Doenças Infecciosas, 2965 Aguas de Moura, Portugal.
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Summary

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Twelve rickettsial isolates, from Rhipicephalus sanguineus, R. turanicus, Dermacentor marginatus and Hyalomma marginatus, were subjected to genotypic analysis. Amplification of specific DNA sequences, restriction endonuclease digestion of amplified DNA products, and gel electrophoresis were used to identify specific DNA fragment-banding patterns. Five patterns were resolved. Four were homologous with those of previously described rickettsial genotypes, R. conorii, R. slovaca, R. rhipicephali and R. massiliae. The fifth pattern differed by only a single altered restriction endonuclease cleavage site. For the first time in Portugal a widely distributed spectrum of spotted fever group rickettsia was found among potential vector species stressing the need to determine their potential for human and domestic animals infection.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 169 - 178
Copyright
Copyright © Cambridge University Press 1995

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