Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-22T16:54:19.906Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic marker studies of poliovirus: II. Strains isolated from cases of poliomyelitis associated with the administration of live attenuated vaccine

Published online by Cambridge University Press:  15 May 2009

Yvonne E. Cossart
Yvonne E. Cossart
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London, N.W. 9
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Strains of poliovirus were obtained from 13 of the 18 persons in England and Wales with paralytic episodes after administration of oral vaccine in 1962. They have been studied using three marker tests: the R.C.T.40 test, intratypic serodifferentiation and inhibition by dextran sulphate. For comparison a number of strains from subjects with non-paralytic vaccine-associated reactions and from patients with paralytic poliomyelitis not related to vaccine were also tested.

Of the eight patients excreting type 1 strains seven came from South Wales where an outbreak was in progress. They all resemble naturally occurring strains from the outbreak in growing at 39·3° but not at 39·8° C.

Only one subject excreted type 2 virus which was of vaccine type.

The type 3 strains included a series from a family group where a range of results from vaccine to the wild range was obtained. Three other patients with vaccineassociated paralysis excreted type 3 strains with the characteristic of naturally occurring strains.

Type
Research Article
Information
Journal of Hygiene , Volume 65 , Issue 1 , March 1967 , pp. 77 - 84
Copyright
Copyright © Cambridge University Press 1967

References

Cossart, Y. E. (1966). Marker studies of poliovirus. Nature, Lond. 211, 1432.CrossRefGoogle ScholarPubMed
Cossart, Y. E. (1967). Genetic marker studies of poliovirus. I. Natural variation. J. Hyg., Camb. 65, 67.CrossRefGoogle ScholarPubMed
Feldman, R. A., Halquin, A. H. & Gelfand, H. M. (1964). Oral poliovirus vaccination in children, a study suggesting enterovirus interference. Pediatrics, Springfield 33, 526.CrossRefGoogle ScholarPubMed
Furez, J., Armstrong, R. E., Moreau, P. & Nagler, F. P. (1964). Genetic markers of poliovirus strains isolated from paralytic patients prior to and after Sabin vaccination programs. II. Field studies on type 3 strains. Am. J. Hyg. 80, 55.Google Scholar
Galbraith, N. S. (1963). Poliomyelitis surveillance in England and Wales, 1962. Proc. IXth Symposium Europ. Ass. Poliomyelitis and Allied Diseases, Stockholm.Google Scholar
Gelfand, H. M. (1963). Oral vaccine: associated paralytic poliomyelitis, 1962. J. Am. med. Ass. 184, 948.CrossRefGoogle Scholar
Lwoff, A. (1962). The thermosensitive critical event in the viral cycle. Cold Spring Harb. Symp. quant. Biol. 27, 159.CrossRefGoogle ScholarPubMed
Magrath, D. I. (1966). A reconsideration of the rct40 marker test for differentiating strains of type 1 poliovirus. Proc. IXth Symposium Europ. Ass. Poliomyelitis and Allied Diseases, Rome.Google Scholar
Magrath, D. I., Boulger, L. R. & Hartley, F. G. (1964). Strains of poliovirus from individuals fed Sabin vaccine studied by in vitro markers and the monkey neurovirulence test. Proc. Xth Symposium Europ. Ass. Poliomyelitis and Allied Diseases, Warsaw. Unpublished.Google Scholar
Miller, D. L. & Galbraith, N. S. (1965). Surveillance of the safety of oral poliomyelitis vaccine in England and Wales, 1962–4. Br. med. J. ii, 504.CrossRefGoogle Scholar
Pavilanis, V., Lussien, G., Foley, A. R., Charhonnian, J. H., Di Franco, E. & Dubrevil, R. (1964). A study of Sabin type 1 oral vaccine virus during five human passages. Revue can. Biol. 23, 265.Google ScholarPubMed
Wecker, E. (1960). A simple test for serodifferentiation of poliovirus strains within the same type. Virology 10, 376.CrossRefGoogle ScholarPubMed