Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-05T05:34:38.983Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of Escherichia coli from porcine postweaning diarrhoea

Published online by Cambridge University Press:  15 May 2009

D. J. Hampson ,
J. M. Woodward  and
I. D. Connaughton
D. J. Hampson
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
J. M. Woodward
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
I. D. Connaughton
Affiliation:
Regional Veterinary Laboratory, Department of Food and Agriculture, Bendigo, Victoria, 3550, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A total of 79 Australian isolates of beta-haemolytic Escherichia coli from cases of porcine postweaning diarrhoea (PWD), and 18 isolates of serotype O 149:K91:K88 (F4) from unweaned pigs from Australia, Indonesia and Denmark, were examined by multilocus enzyme electrophoresis. These were divided into 57 electrophoretic types (ETs), with an overall mean genetic diversity per enzyme locus of 0·466. This value closely resembled that previously recorded for the whole species. Not only was the collection diverse, but there was considerable genetic heterogeneity amongst PWD isolates of the same serogroup. Isolates from serogroups O 8 and O 138 were most varied, whilst many from serogroups O 141 and O 149 were more closely related. In contrast, the isolates from the unweaned pigs all belonged to only one ET.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 3 , June 1993 , pp. 575 - 581
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Richards, WPC, Fraser, CM. Coliform enteritis of weaned pigs. A description of the disease and its association with hemolytic Escherichia coli. Cornell Vet 1961; 51: 245–57.Google ScholarPubMed
2.Sojka, WJ. In ‘Escherichia coli in domestic animals and poultry’, 1965. Commonwealth Agricultural Bureau, Weybridge, England.Google Scholar
3.Svendsen, J. Enteric Escherichia coli diseases in weaned pigs. Nord VetMed 1974: 26: 226–38.Google ScholarPubMed
4.Dam, A, Knox, B. Haemolytic Escherichia coli associated with enteritis and enterotoxaemia in pigs in Denmark, with particular reference to the rapid spread of serogroup O 149:K91. Nord VetMed 1974; 26: 219–25.Google Scholar
5.Bertschinger, HU, Jucker, H, Pfirter, HP. Control of Escherichia coli infection in weaned pigs by use of oral immunization combined with a diet low in nutrients. Fortschr VetMed 1979: 29: 73–81.Google Scholar
6.Smith, HW, Linggood, MA. Observations on the pathogenic properties of the K88. hly and ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. J Med Microbiol 1971; 4: 467–85.CrossRefGoogle ScholarPubMed
7.Selander, RK, Caugant, DA, Ochman, H, Musser, JM, Gilmour, MN, Whittam, TS. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 1986; 51: 873–84.CrossRefGoogle ScholarPubMed
8.Nei, M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978; 89: 583–90.CrossRefGoogle ScholarPubMed
9.Caugant, DA, Levin, BR, Orskov, I, Orskov, F, Eden, CS, Selander, RK. Genetic diversity in relation to serotype in Escherichia coli. Infect Immun 1985; 49: 407–13.CrossRefGoogle ScholarPubMed
10.Sneath, PHA. Sakal, RR. Numerical taxonomy. San Francisco: CA: WH Freeman 1973: 573.Google Scholar
11.Selander, RK. Levin, BR. Genetic diversity and structure in Escherichia coli populations. Science 1980; 210: 545–7.CrossRefGoogle ScholarPubMed
12.Kuhn, I. Franklin, A, Soderlind, O, Mollby, R. Phenotypic variations among enterotoxigenic Escherichia coli from Swedish piglets with diarrhoea. Med Microbiol Immunol 1985; 174: 119–30.CrossRefGoogle Scholar
13.Vasenius, H. The influence of dietary carbohydrate on the multiplication and colonization of inoculated Escherichia coli strains in pigs intestines. II. Investigations in pigs at weaning age. Nord VetMed 1969: 21: 535–44.Google Scholar
14.Larsen, JL. Differences between enteropathogenic Escherichia coli strains isolates from neonatal E. coli diarrhoea (NCD) and postweaning diarrhoea (PWD) in pigs. Nord VetMed 1976: 28: 417–29.Google Scholar
15.Hoblet, KH, Kohler, EM. Saif, LH, Theil, KW, Ingalls, WL. Study of porcine post-weaning diarrhoea involving K88 (-) hemolytic Escherichia coli. Am J Vet Res 1986; 47: 1910–2.Google Scholar
16.Soderlind, O, Thafvelin, B, Mollby, R. Virulence factors in Escherichia coli strains isolated from Swedish piglets with diarrhea. J Clin Microbiol 1988; 26: 879–84.CrossRefGoogle ScholarPubMed
17.Kennan, RM. Monckton, RP. Adhesive fimbriae associated with porcine enterotoxigenic Escherichia coli of the O 141 serotype. J Clin Microbiol 1990; 28: 2006–11.CrossRefGoogle Scholar
18.Bertschinger, HU. Bachman, M, Mettler, C, et al. Adhesive fimbriae produced in vivo by Escherichia coli O 139:K12 (B):H1 associated with entertoxaemia in pigs. Vet Microbiol 1990: 25: 267–81.CrossRefGoogle Scholar
19.Nagy, B. Casey, TA, Whipp, SC, Moon, HW. Susceptibility of porcine intestine to pilusmediated adhesion by some isolates of piliated enterotoxigenic Escherichia coli increases with age. Infect Immun 1992; 60: 1285–94.CrossRefGoogle ScholarPubMed
20.Salajka, E. Salajkova, Z. Alexa, P, Hornich, M. Colonization factor different from K88, K99, F41 and 987P in enterotoxigenic Escherichia coli strains isolated from postweaning diarrhoea in pigs. Vet Microbiol 1992; 32: 163–75.CrossRefGoogle ScholarPubMed