Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2025-01-05T11:44:32.029Z Has data issue: false hasContentIssue false
  • English
  • Français

Further experiments with the soluble antigen of the MEF1 poliomyelitis virus

Published online by Cambridge University Press:  15 May 2009

G. Selzer  and
M. van den Ende
G. Selzer
Affiliation:
The C.S.I.R. and U.C.T. Virus Research Unit, Department of Pathology, University of Cape Town
M. van den Ende
Affiliation:
The C.S.I.R. and U.C.T. Virus Research Unit, Department of Pathology, University of Cape Town
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

By ultracentrifugation of emulsions of brains from suckling mice infected with the MEF1 strain of poliomyelitis virus, infective virus can be separated from a non-infective soluble antigen. The soluble antigen which remains in the supernatant fluid is serologically specific and is responsible for most of the complement fixation shown by such brain emulsions.

Soluble antigen is not demonstrable in the brains of adult mice infected with a strain of the virus which has not been adapted to sucklings.

The soluble antigen is heat stable and resists treatment with organic lipoid solvents such as acetone, ether and chloroform.

Type
Research Article
Information
Journal of Hygiene , Volume 54 , Issue 1 , March 1956 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1956

References

REFERENCES

Casals, J. (1949). Acetone-ether extracted antigens for complement fixation with certain neurotropic viruses. Proc. Soc. exp. Biol., N.Y., 70, 339.CrossRefGoogle ScholarPubMed
Casals, J. & Olitsky, P. K. (1950). A complement fixation test for poliomyelitis virus. Proc. Soc. exp. Biol., N.Y., 75, 315.CrossRefGoogle ScholarPubMed
Casals, J., Olitsky, P. K. & Anslow, R. O. (1951). A specific complement fixation test for infection with poliomyelitis virus. J. exp. Med. 94, 123.CrossRefGoogle Scholar
Henle, W. (1953). Advances in Virus Research, p. 161. New York: Academic Press Inc.Google Scholar
Reed, L. & Meunch, H. (1938). Simple method of estimating 50 per cent endpoints. Amer. J. Hyg. 27, 493.Google Scholar
Rustigian, R., Havens, P. & Kolner, M. (1954). Possible occurrence of multiple antigens in Type 2 poliomyelitis and GDVII mouse encephalomyelitis viruses. Proc. Soc. exp. Biol., N.Y., 86, 500.CrossRefGoogle ScholarPubMed
Selzer, G. & Polson, A. (1954). Dispersion analysis of the MEF1 strain of poliomyelitis virus. Biochim. biophys. Acta, 15, 251.Google Scholar
Selzer, G., Sacks, M. & van den Ende, M. (1952). Adaptation and multiplication rate of the MEF1 strain of poliomyelitis virus in new-born mice. S.A. med. J. 26, 201.Google Scholar