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The fate of 131I-labelled diphtheria toxin and toxoid in the skin of immune and allergic guinea-pigs*

Published online by Cambridge University Press:  15 May 2009

D. A. Long
D. A. Long
Affiliation:
From the Department of Microbiology, School of Medicine, University of Pittsburgh, PA
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Highly purified crystalline diphtheria toxin-protein, and toxoid prepared from it, were labelled with 131I, without change of potency, and injected intradermally into immune and allergic guinea-pigs. It is probable that actively immunized guinea-pigs localize these antigens and that localization is due to hypersensitivity of a type that cannot be transferred with serum; it is possibly of the delayed (tuberculin-type) of allergy.

It is a pleasure to acknowledge the gift of highly purified (crystalline) diphtheria toxin, and toxoid prepared from it, from Dr C. G. Pope of the Wellcome Research Laboratories, England. Dr S. P. Masouredis of the Central Blood Bank of Pittsburgh kindly labelled these antigens, using the technique he devised. Dr W. J. Kuhns generously provided the human precipitating and non-precipitating diphtheria antitoxin*.

The collimator head was made by Mr J. Nechaj of the University of Pittsburgh.

All these collaborators gave me advice on matters of which I was ignorant; for this I am grateful.

Type
Research Article
Information
Journal of Hygiene , Volume 57 , Issue 2 , June 1959 , pp. 227 - 234
Copyright
Copyright © Cambridge University Press 1959

References

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