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Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents)

Published online by Cambridge University Press:  15 May 2009

F. W. A. Johnson  and
D. Hobson
F. W. A. Johnson
Affiliation:
Department of Medical Microbiology, University of Liverpool
D. Hobson
Affiliation:
Department of Medical Microbiology, University of Liverpool
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Summary

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Normal non-irradiated McCoy cell cultures provide a sensitive and reproducible method for the isolation of oculo-genital strains of chlamydia A directly from human secretions and for laboratory studies with these agents. Since September 1973, chlamydia have been isolated from 175 of 562 women (32·1%) attending venereal disease clinics. Freshly isolated and low passage strains have been used to determine the importance of centrifugation, constitution and pH of the tissue culture medium, and the temperature of incubation in controlling the efficiency of plating in the method.

Type
Research Article
Information
Journal of Hygiene , Volume 76 , Issue 3 , June 1976 , pp. 441 - 451
Copyright
Copyright © Cambridge University Press 1976

References

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