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Experimental studies on the infectivity of non-culturable forms of Campylobacter spp. in chicks and mice

Published online by Cambridge University Press:  15 May 2009

A. W. Van De Giessen ,
C. J. Heuvelman ,
T. Abee  and
W. C. Hazeleger
A. W. Van De Giessen*
Affiliation:
Microbiological Laboratory for Health Protection, National Institute of Public Health and Environmental Protection, P.O. Box No. 1, 3720 BA Bilthoven, The Netherlands
C. J. Heuvelman
Affiliation:
Microbiological Laboratory for Health Protection, National Institute of Public Health and Environmental Protection, P.O. Box No. 1, 3720 BA Bilthoven, The Netherlands
T. Abee
Affiliation:
Department of Food Science, Wageningen Agricultural University, Wageningen, The Netherlands
W. C. Hazeleger
Affiliation:
Department of Food Science, Wageningen Agricultural University, Wageningen, The Netherlands
*
* Author for correspondence.
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The significance of non-culturable forms of Campylobacter spp., especially with regard to the epidemiology of this organism in poultry flocks, was explored. Two different experiments were conducted to produce non-culturable Campylobacter spp. and test their ability to colonize the animal gut. In the first experiment a mixture of 28 different strains of Campylobacter spp. from various sources was inoculated in both sterilized surface water and potassium phosphate buffer and stored at 4 °C. After Campylobacter spp. were no longer detectable by culture in the microcosms, the mixtures of non-culturable cells were used to challenge both chicks and mice. Recovery of non-culturable Campylobacter spp. from the animals was not successful at 4 weeks after administration. In the second experiment the survival of six individual strains of Campylobacter spp. in sterilized surface water at 4 °C was studied and the resulting non-culturable cells were used to challenge chicks. None of the Campylobacter strains could be recovered from the chicks at 2 weeks after administration. We conclude that occurrence of non-culturable forms of Campylobacter spp. capable of colonizing chicks is not a common phenomenon and that non-culturable forms of Campylobacter spp. are likely to be insignificant for importantly to the epidemiology of the organism in Dutch broiler flocks.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 3 , December 1996 , pp. 463 - 470
Copyright
Copyright © Cambridge University Press 1996

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