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Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis: a preliminary investigation

Published online by Cambridge University Press:  15 May 2009

P. F. Rae ,
M. V. Thrusfield ,
A. Higgins ,
C. G. G. Aitken ,
T. W. Jones  and
A. G. Luckins
P. F. Rae
Affiliation:
Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, EH25 9RG
M. V. Thrusfield
Affiliation:
Department of Veterinary Clinical Studies, University of Edinburgh, Royal (Dick) School of Veterinary Studies, Veterinary Field Station, Easter Bush, Roslin, Midlothian, EH25 9RG
A. Higgins
Affiliation:
Aid Section, The British Embassy, P.O. Box 801, Khartoum, Sudan
C. G. G. Aitken
Affiliation:
Department of Statistics, University of Edinburgh, James Clerk Maxwell Building, The King's Buildings, Edinburgh, EH9 3JZ
T. W. Jones
Affiliation:
Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, EH25 9RG
A. G. Luckins
Affiliation:
Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, EH25 9RG
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Three enzyme immunoassays were used for the serodiagnosis of Trypanosoma evansi in camels in the Sudan in order to evaluate their ability to discriminate between infected and non-infected animals. Two assays were used for the detection of trypanosomal antibodies, one using specific anti-camel IgG conjugate and another using a non-specific Protein A conjugate. The third assay detected the presence of trypanosomal antigens using anti-T. evansi antibodies in a double antibody sandwich assay. Inspection of the frequency distribution of assay results suggested that the ELISA for circulating trypanosomal antibodies using specific antisera and the ELISA for circulating antigens can distinguish between non-infected camels and infected camels exhibiting patent infections or not. The ELISA using Protein A conjugate to bind non-specifically to camel immunoglobulin did not appear to discriminate between infected and non-infected animals.

Type
Research Article
Information
Epidemiology & Infection , Volume 102 , Issue 2 , April 1989 , pp. 297 - 307
Copyright
Copyright © Cambridge University Press 1989

References

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