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Esterase electrophoresis: a molecular tool for studying the epidemiology of Branhamella catarrhalis nosocomial infection

Published online by Cambridge University Press:  15 May 2009

B. Picard ,
PH. Goullet ,
E. Denamur  and
G. Suermondt
B. Picard
Affiliation:
Service de Microbiologie, Hôpital Beaujon (Centre Hospitalier Bichat-Beaujon, Université Paris VII), 100 boulevard du Général Leclerc, 92110 Clichy, France
PH. Goullet
Affiliation:
Service de Microbiologie, Hôpital Beaujon (Centre Hospitalier Bichat-Beaujon, Université Paris VII), 100 boulevard du Général Leclerc, 92110 Clichy, France
E. Denamur
Affiliation:
Service de Microbiologie, Hôpital Nord, Place Victor Pauchet, 80030 Amiens Cédex, France
G. Suermondt
Affiliation:
Service de Microbiologie, Hôpital Nord, Place Victor Pauchet, 80030 Amiens Cédex, France
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Summary

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A new epidemiologic typing method based on electrophoresis of esterases had been developed for differentiating between clinical isolates of Branhamella catarrhalis. Twenty-two epidemiologically significant strains obtained from three Chest Units, a Paediatric Intensive Care Unit and a Paediatric Unit were compared with 54 randomly selected strains and 4 reference strains, including the species type strain, ATCC 25238. Thirty-four distinct zymotypes were characterized by polyacrylamide-agarose gel electrophoresis of the 80 strains. One infrequent zymotype was found in 2 neonates and another in 2 adults with nosocomial bronchopulmonary infections, suggesting the nosocomial spread of 2 outbreak strains of B. catarrhalis. A more frequent zymotype was isolated from 3 neonates with nosocomial bronchopulmonary infection and from 2 children with nosocomial rhinopharyngitis. The remaining 12 epidemiologically significant strains were of varied zymotypes. This work demonstrates that esterase electrophoresis is a suitable, readily reproducible, stable typing system applicable to the wide range of strains found in B. catarrhalis nosocomial infections.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 547 - 554
Copyright
Copyright © Cambridge University Press 1989

References

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