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Epidemiology of Staphylococcus aureus in patients with cystic fibrosis

Published online by Cambridge University Press:  15 May 2009

C. Branger ,
J. M. Fournier ,
J. Loulergue ,
A. Bouvet ,
Ph. Goullet ,
A. Boutonnier ,
C. De Gialluly ,
G. Couetdic ,
M. Chomarat  and
M. C. Jaffar-Banjee
...Show all authors
C. Branger*
Affiliation:
Laboratoire de Microbiologie, Faculté de Médecine X. Bichat, Paris VII
J. M. Fournier
Affiliation:
Unité du Choléra et des Vibrions, Institut Pasteur, Paris
J. Loulergue
Affiliation:
Laboratoire de Bactériologie. Hôpital Trousseau, Tours
A. Bouvet
Affiliation:
Laboratoire de Microbiologie, Hôpital Hôtel-Dieu, Paris
Ph. Goullet
Affiliation:
Laboratoire de Microbiologie, Faculté de Médecine X. Bichat, Paris VII
A. Boutonnier
Affiliation:
Unité du Choléra et des Vibrions, Institut Pasteur, Paris
C. De Gialluly
Affiliation:
Laboratoire de Bactériologie. Hôpital Trousseau, Tours
G. Couetdic
Affiliation:
Laboratoire de Microbiologie, Hôpital J. Minjoz, Besançon
M. Chomarat
Affiliation:
Laboratoire de Bactériologie, Centre Hospitalier Lyon Sud, Pierre-Benite
M. C. Jaffar-Banjee
Affiliation:
Laboratoire de Biochimie-Bactériologie, Hôpital Renée Sabran, Giens, Hyères
P. Mariani
Affiliation:
Laboratoire de Microbiologie, Hôpital Robert Debré, Paris, France
*
* Correspondence and reprint requests: Dr Catherine Branger, Laboratoire de Microbiologie, Hopital Beaujon. 100 Boulevard du Général Leclerc, 92110 Clichy, France.
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Seven hundred and thirty-four isolates of Staphylococcus aureus, recovered from the sputum of 238 cystic fibrosis patients in six French hospitals, were characterized by esterase electrophoretic typing, capsular polysaccharide serotyping and phage typing and tested against 14 antibiotics for sensitivity. Thirty-four esterase electrophoretic types were found with a genotypic diversity coefficient of 0·91. Five hundred and forty-eight (78·7%) isolates produced capsular polysaccharide and 350 (50·3%) were type 8. Four hundred and sixty isolates (66·6%) were phage typable and 202 (28·2%) were lysed by group III bacteriophages. No esterase electrophoretic type, capsular type or phage type was specific to cystic fibrosis. Isolates belonged to a wide range of types, similar to strains acquired outside hospitals. Eighty-five patients had three or more consecutive isolates over at least 6 months. The ability of S. aureus to persist for long periods of time has been demonstrated in 73% of them. Methicillin-resistance was encountered among 73 strains (9·8%) which were also multiresistant. Two hundred and eighty-nine (39·9%) strains were sensitive to all antibiotics tested except to penicillin. Pristinamycin and co-trimoxazole were the most effective antibiotics. These results could contribute to the elaboration of a rational approach to the prophylaxis and therapy of respiratory staphylococcal infections in cystic fibrosis patients.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 489 - 500
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Bauernfeind, A, Bertele, RM, Harms, K et al. , Qualitative and quantitative microbiological analysis of sputa of 102 patients with cystic fibrosis. Infect 1987; 15: 270–7.CrossRefGoogle ScholarPubMed
2.Friend, PA. Pulmonary infection in cystic fibrosis. J Infect 1986; 13: 55–72.CrossRefGoogle ScholarPubMed
3.Gilligan, PH. Microbiology of airway disease in patients with cystic fibrosis. Clin Microbiol Rev 1991: 4: 35–51.CrossRefGoogle ScholarPubMed
4.Hoiby, N. Haemophilus influenzae, Staphylococcus aureus, Pseudomonas cepacia and Pseudomonas aeruginosa in patients with cystic fibrosis. Chest 1988; 94: 97–103.CrossRefGoogle ScholarPubMed
5.Bauernfeind, A, Przyklenk, B, Matthias, C, Jungwirth, R, Bertele, RM, Harms, K. Selection of antibiotics for treatment and prophylaxis of staphyloeoccal infections in cystic fibrosis patients. Infect 1990; 18: 126–30.CrossRefGoogle ScholarPubMed
6.Hoff, GE, Hoiby, X. Staphylococcus aureus in cystic fibrosis: antibiotic sensitivity and phage types during the latest decade. Acta Path Microbiol Scand 1975; 83: 219–25.Google ScholarPubMed
7.Marks, MI. Clinical significance of Staphylococcus aureus in cystic fibrosis. Infect 1990: 18: 53–6.CrossRefGoogle ScholarPubMed
8.Guggenbichler, JP, Schmeeberger, J. Antimicrobial chemotherapy in patients with cystic fibrosis. Infect 1987; 15: 397–402.CrossRefGoogle ScholarPubMed
9.Goering, RV, Bauernfeind, A, Lenz, W, Przyklenk, B. Staphylococcus aureus in patients with cystic fibrosis: an epidemiological analysis using a combination of traditional and molecular methods. Infect 1990; 18: 57–60.CrossRefGoogle ScholarPubMed
10.Hoiby, N. Epidemiological investigations of the respiratory tract bacteriology in patients with cystic fibrosis. Acta Path Microbiol Scand 1974: 82: 541–50.Google ScholarPubMed
11.Bouvet, A, Fournier, JM, Audurier, A, Branger, C, Orsoni, A, Girard, C. Epidemiological markers for epidemic strains and carrier isolates in an outbreak of nosocomial oxacillin-resistant Staphylococcus aureus. J Clin Microbiol 1990; 28: 1338–41.CrossRefGoogle Scholar
12.Branger, C, Goullet, P. Esterase electrophoretic polymorphism of methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus. J Med Microbiol 1987; 24: 275–81.CrossRefGoogle ScholarPubMed
13.Branger, C, Goullet, P. Genetic heterogeneity in methicillin-resistant strains of Staphylococcus aureus revealed by esterase electrophoretic polymorphism. J Hosp Infect 1989; 14: 125–34.CrossRefGoogle ScholarPubMed
14.Arbeit, RD, Karakawa, WW, Vann, WF, Robbins, JB. Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus. Diagn Microbiol Infect Dis 1984; 2: 85–91.CrossRefGoogle ScholarPubMed
15.Hochkeppel, HK, Braun, DG, Vischer, W et al. , Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolates with monoclonal antibodies to capsular polysaccharide types 5 and 8. J Clin Microbiol 1987; 25: 526–30.CrossRefGoogle ScholarPubMed
16.Karakawa, WW, Fournier, JM, Vann, WF, Arbeit, R, Schneerson, RS, Robbins, JB. Method for serological typing of the capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol 1985; 22: 445–7.CrossRefGoogle ScholarPubMed
17.Sompolinsky, D, Samra, Z, Karakawa, WW, Vann, WF, Schneerson, R, Malik, Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol 1985; 22: 828–34.CrossRefGoogle ScholarPubMed
18.Albus, A, Fournier, JM, Wolz, C et al. Staphylococcus aureus capsular types and antibody response to lung infection in patients with cystic fibrosis. J Clin Microbiol 1988: 26: 2505–9.CrossRefGoogle ScholarPubMed
19.Blair, JE, Williams, REO. Phage typing of staphylococci. Bull WHO 1961; 24: 771–84.Google ScholarPubMed
20.Goullet, P. An esterase zymogram of Escherichia coli. J Gen Microbiol 1973; 77: 27–35.CrossRefGoogle ScholarPubMed
21.Boutonnier, A, Nato, F, Bouvet, A et al. , Direct testing of blood cultures for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol 1989: 27:989–93.CrossRefGoogle ScholarPubMed
22.Nei, M. Estimation of average heterozygosity and genetic distance from a small sample of individuals. Genetics 1978; 89: 583–90.CrossRefGoogle ScholarPubMed
23.Selander, RK, Caugant, DA, Ochman, H, Musser, JM, Gilmour, MX, Whittam, TS. Methods of multiloeus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 1986; 51: 873–84.CrossRefGoogle ScholarPubMed
24.Acar, J, Bergogne-Bérézin, E, Brogard, JM et al. Communiqué du Comité de 1'Anti-biogramme de la Société Française de Microbiologie. Pathol Biol 1993: 41: 741–8.Google Scholar
25.Branger, C, Goullet, P, Boutonnier, A, Fournier, JM. Correlation between esterase electrophoretic types and capsular polysaccharide types 5 and 8 among methicillin-susceptible and methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol 1990: 28: 150–1.CrossRefGoogle ScholarPubMed
26.Schlichting, C, Branger, C, Fournier, JM et al. Typing of Staphylococcus aureus by pulsed field gel electrophoresis, zymotyping, capsular typing, and phage typing: resolution of clonal relationships. J Clin Microbiol 1993: 31: 227–32.CrossRefGoogle ScholarPubMed
27.Fournier, JM. Staphylococcus aureus. In: Cryz, SJ, ed. Vaccines and immunotherapy. New York: Pergamon Press. 1991: 166–77.Google Scholar