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Enzyme immunoassay using BCG in serodiagnosis of pulmonary tuberculosis

Published online by Cambridge University Press:  19 October 2009

F. L. Garcia-Carreñno ,
R. E. Carvajal  and
R. Hernandez
F. L. Garcia-Carreñno
Affiliation:
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Apartado Postal 2-191, 44270 Guadalajara, Jalisco, Mexico
R. E. Carvajal
Affiliation:
Departamento de Bioquimica, Facultad de Medicina, UNAM
R. Hernandez
Affiliation:
Unidad de Investigación en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, C.M.N.
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Summary

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Amounts of Mycobacterium tuberculosis antibodies were determined in sera from patients with either active or inactive tuberculosis and in healthy subjects by an immunoenzymatic assay in which whole BCG cells attached covalently to polystyrene disks were used as antigen. Statistically significant differences (P < 0·005) were found both between the active and inactive tuberculosis groups and between the active group and healthy controls. No significant differences were found between the inactive group and controls. Since this procedure is efficient (91%) and can be used in areas which lack laboratory equipment, it appears promising for individual serodiagnosis and for epidemiological surveys.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 3 , December 1986 , pp. 483 - 487
Copyright
Copyright © Cambridge University Press 1986

References

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