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Environmental isolates of Citrobacter braakii that agglutinate with Escherichia coli O157 antiserum but do not possess the genes responsible for the biosynthesis of O157 somatic antigen

Published online by Cambridge University Press:  29 April 2003

A. KHAN ,
R. K. NANDI ,
S. C. DAS ,
T. RAMAMURTHY ,
J. KHANAM ,
T. SHIMIZU ,
S. YAMASAKI ,
S. K. BHATTACHARYA ,
W. CHAICUMPA  and
Y. TAKEDA
...Show all authors
A. KHAN
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
R. K. NANDI
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
S. C. DAS
Affiliation:
Indian Veterinary Research Institute, Belgachia, Calcutta, India
T. RAMAMURTHY
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
J. KHANAM
Affiliation:
Jadavpur University, Jadavpur, Calcutta, India
T. SHIMIZU
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Japan
S. YAMASAKI
Affiliation:
Department of Veterinary Sciences, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Japan
S. K. BHATTACHARYA
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
W. CHAICUMPA
Affiliation:
Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
Y. TAKEDA
Affiliation:
Faculty of Human Life Sciences, Jissen Women's University, Tokyo 181-8510, Japan
G. BALAKRISH NAIR
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) Dhaka, Bangladesh
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Abstract

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While searching for Escherichia coli O157 in the aquatic environment of Calcutta using an immunodetection procedure, we fortuitously detected five strains of Citrobacter braakii, which cross-reacted with the commercially available O157 polyvalent antiserum. The five C. braakii isolates gave positive results when a sensitive dot-ELISA was performed with E. coli O157 monoclonal antibody. Further, the O157 monoclonal antibody recognized the bands of proteinase K treated whole cells of lipopolysaccharide of all the C. braakii isolates. Apart from weak reactions with two or three of the DNA probes, all the C. braakii strains did not hybridize with the other probes spanning the minimum region required for O157 O-antigen biosynthesis. These strains did not possess any of the virulence genes that are commonly found in the Shiga toxin-producing E. coli (STEC) specially the serotype O157[ratio ]H7. Therefore, it appears that the serological cross-reaction between C. braakii and E. coli O157 antiserum is based on structural mimicry between the O-polysaccharide of C. braakii and E. coli O157.

Type
Research Article
Information
Epidemiology & Infection , Volume 130 , Issue 2 , April 2003 , pp. 179 - 186
Copyright
© 2003 Cambridge University Press